Human SPA17 Recombinant Protein (RPPB4796)

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RPPB4796

Description

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Product Name:Human SPA17 Recombinant Protein
Product Code:RPPB4796
Size:10µg
Species:Human
Target:SPA17
Synonyms:CT22, SP17, SP17-1, Sperm surface protein Sp17, Cancer/testis antigen 22, Sperm autoantigenic protein 17, Sperm protein 17, SPA17.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SPA17 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK

SPA17 is a protein present at the cell surface. SPA17 was characterized by its participation in the binding of sperm to the zona pellucida of the oocyte. The central portion of SPA17 has carbohydrate binding motifs and likely takes part in cell-cell adhesion functions such as immune cell migration and metastasis.

SPA17 Human Recombinant produced in E. coli is a single polypeptide chain containing 174 amino acids (1-151) and having a molecular mass of 19.8kDa. SPA17 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SPA17: Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.
UniProt Protein Details:

Protein type:Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: membrane; cytoplasm

Molecular Function:calmodulin binding

Biological Process: binding of sperm to zona pellucida; single fertilization; spermatogenesis

NCBI Summary:This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.[provided by RefSeq, Jan 2009]
UniProt Code:Q15506
NCBI GenInfo Identifier:2833264
NCBI Gene ID:53340
NCBI Accession:Q15506.1
UniProt Secondary Accession:Q15506,Q9BXF7, B2R4F2,
UniProt Related Accession:Q15506
Molecular Weight:151
NCBI Full Name:Sperm surface protein Sp17
NCBI Synonym Full Names:sperm autoantigenic protein 17
NCBI Official Symbol:SPA17��
NCBI Official Synonym Symbols:CT22; SP17; SP17-1��
NCBI Protein Information:sperm surface protein Sp17; sperm protein 17; cancer/testis antigen 22
UniProt Protein Name:Sperm surface protein Sp17
UniProt Synonym Protein Names:Cancer/testis antigen 22; CT22; Sp17-1; Sperm autoantigenic protein 17; Sperm protein 17
Protein Family:Sperm surface protein
UniProt Gene Name:SPA17��
UniProt Entry Name:SP17_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15506
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