Human SNRPC Recombinant Protein (RPPB4752)

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RPPB4752

Description

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Product Name:Human SNRPC Recombinant Protein
Product Code:RPPB4752
Size:5µg
Species:Human
Target:SNRPC
Synonyms:U1 small nuclear ribonucleoprotein C, U1 snRNP C, U1-C, U1C, SNRPC, Yhc1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:SNRPC protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 50% glycerol, 0.3M NaCl, 5mM DTT and 2mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMPKFYCD YCDTYLTHDS PSVRKTHCSG RKHKENVKDY YQKWMEEQAQ SLIDKTTAAF QQGKIPPTPF SAPPPAGAMI PPPPSLPGPP RPGMMPAPHM GGPPMMPMMG PPPPGMMPVG PAPGMRPPMG GHMPMMPGPP MMRPPARPMM VPTRPGMTRP DR

SNRPC is a member of the U1 small nuclear ribonucleoprotein C family. The SNRPC protein component of the U1 small nuclear ribonucleoprotein (snRNP) particle required for the formation of the spliceosome. SNRPC participates in the processing of nuclear precursor messenger RNA splicing. snRNP particles are tackled by autoantibodies frequently produced by patients with connective tissue diseases.

SNRPC Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-159 a.a.) and having a molecular mass of 19.8kDa (Molecular weight on SDS-PAGE will appear higher).SNRPC is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:snRNP C: Component of the U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1-C is directly involved in initial 5' splice-site recognition for both constitutive and regulated alternative splicing. The interaction with the 5' splice-site seems to precede base-pairing between the pre-mRNA and the U1 snRNA. Stimulates E complex formation by stabilizing the base pairing of the 5' end of the U1 snRNA and the 5' splice-site region. Belongs to the U1 small nuclear ribonucleoprotein C family.
UniProt Protein Details:

Protein type:RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: Cajal body; nucleoplasm; snRNP U1

Molecular Function:pre-mRNA 5'-splice site binding; protein binding; protein homodimerization activity; single-stranded RNA binding; U1 snRNA binding; zinc ion binding

Biological Process: nuclear mRNA 5'-splice site recognition; nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis

NCBI Summary:This gene encodes one of the specific protein components of the U1 small nuclear ribonucleoprotein (snRNP) particle required for the formation of the spliceosome. The encoded protein participates in the processing of nuclear precursor messenger RNA splicing. snRNP particles are attacked by autoantibodies frequently produced by patients with connective tissue diseases. The genome contains several pseudogenes of this functional gene. Alternative splicing results in a non-coding transcript variant.[provided by RefSeq, Oct 2009]
UniProt Code:P09234
NCBI GenInfo Identifier:134093
NCBI Gene ID:6631
NCBI Accession:P09234.1
UniProt Secondary Accession:P09234,Q5TAL3,
UniProt Related Accession:P09234
Molecular Weight:17,394 Da
NCBI Full Name:U1 small nuclear ribonucleoprotein C
NCBI Synonym Full Names:small nuclear ribonucleoprotein polypeptide C
NCBI Official Symbol:SNRPC��
NCBI Official Synonym Symbols:U1C; Yhc1��
NCBI Protein Information:U1 small nuclear ribonucleoprotein C
UniProt Protein Name:U1 small nuclear ribonucleoprotein C
UniProt Gene Name:SNRPC��
UniProt Entry Name:RU1C_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P09234
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