Human SNF8 Recombinant Protein (RPPB4743)

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SKU:
RPPB4743
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96H20

Description

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Product Name:Human SNF8 Recombinant Protein
Product Code:RPPB4743
Size:20µg
Species:Human
Target:SNF8
Synonyms:SNF8 ESCRT-II complex subunit homolog (S. cerevisiae), ESCRT-II complex subunit VPS22, ELL-associated protein of 30 kDa, EAP30 subunit of ELL complex, vacuolar-sorting protein SNF8, EAP30, VPS22, Dot3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SNF8 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 2mM DTT and 50% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMHRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT� GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP

SNF8 belongs to the SNF8 family of vacuolar sorting proteins and is restricts to both the nucleus and the cytoplasm. SNF8 is a subunit of the endosomal sorting complex essential for transport II (ESCRT-II), which is necessary for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway facilitates transfer of transmembrane proteins into the lumen of the lysosome for degradation. Additionally, the ESCRT-II complex takes part in transcription regulation by contributing to derepression of transcription by RNA polymerase II, probably by its interface with ELL.

SNF8 Human Recombinant produced in E. coli is a single polypeptide chain containing 282 amino acids (1-258) and having a molecular mass of 31.4 kDa.SNF8 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SNF8: Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Belongs to the SNF8 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; cytosol; endosome membrane; late endosome membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; recycling endosome; transcription factor complex

Molecular Function:channel regulator activity; protein binding; protein C-terminus binding; protein homodimerization activity; protein N-terminus binding; transcription factor binding

Biological Process: early endosome to late endosome transport; endocytic recycling; endosome transport; macroautophagy; positive regulation of protein catabolic process; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; regulation of MAP kinase activity; regulation of protein catabolic process; regulation of transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
UniProt Code:Q96H20
NCBI GenInfo Identifier:73919323
NCBI Gene ID:11267
NCBI Accession:Q96H20.1
UniProt Secondary Accession:Q96H20,Q8IXY3, Q9UN50,
UniProt Related Accession:Q96H20
Molecular Weight:29kDa
NCBI Full Name:Vacuolar-sorting protein SNF8
NCBI Synonym Full Names:SNF8, ESCRT-II complex subunit
NCBI Official Symbol:SNF8��
NCBI Official Synonym Symbols:Dot3; EAP30; VPS22��
NCBI Protein Information:vacuolar-sorting protein SNF8
UniProt Protein Name:Vacuolar-sorting protein SNF8
UniProt Synonym Protein Names:ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22
Protein Family:Vacuolar-sorting protein
UniProt Gene Name:SNF8��
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