Human SNAPIN Recombinant Protein (RPPB4727)

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RPPB4727

Description

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Product Name:Human SNAPIN Recombinant Protein
Product Code:RPPB4727
Size:50µg
Species:Human
Target:SNAPIN
Synonyms:SNAPAP, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein, SNAP-associated protein, SNAPIN, SNAP25BP.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The protein solution contains 20mM Tris-HCl pH-8, 5mM DTT, 2mM EDTA, 0.2M NaCl, and 40% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAGAGSAAVS GAGTPVAGPT GRDLFAEGLL EFLRPAVQQL DSHVHAVRES QVELREQIDN LATELCRINEDQKVALDLDP YVKKLLNARR RVVLVNNILQ NAQERLRRLN HSVAKETARR RAMLDSGIYP PGSPGK

SNAPIN is involved in the neurotransmitter release process through its modulation of the sequential interactions between the SNAREs and synaptotagmin. SNAPIN is part of the SNARE complex of proteins that is needed for synaptic vesicle docking and fusion. SNAPAP is enriched in neurons and exclusively located on synaptic vesicle membrane protein. SNAPIN is also an important factor of the BLOC1 multisubunit protein complex. BLOC1 is required for normal biogenesis of specialized organelles of the endosomal-lysosomal system, such as melanosomes and platelet dense granules.

SNAPIN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-136) and having a molecular mass of 17 kDa. SNAPIN is fused to 20 amino acid His Tag at N-terminus and purified by standard chromatography techniques.

UniProt Protein Function:SNAPIN: a component of the SNARE complex of proteins that is required for synaptic vesicle docking and fusion. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. Phosphorylation by PKA modulates its interaction with the SNARE complex.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: perinuclear region of cytoplasm; secretory granule; synapse; synaptic vesicle

Molecular Function:protein binding; SNARE binding

Biological Process: anterograde axon cargo transport; anterograde synaptic vesicle transport; endosome to lysosome transport; lysosome localization; neurite development; neurotransmitter secretion; regulation of protein binding; synaptic vesicle exocytosis; synaptic vesicle transport

NCBI Summary:The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
UniProt Code:O95295
NCBI GenInfo Identifier:41017846
NCBI Gene ID:23557
NCBI Accession:O95295.1
UniProt Secondary Accession:O95295,Q5SXU8, D3DV56,
UniProt Related Accession:O95295
Molecular Weight:15kDa
NCBI Full Name:SNARE-associated protein Snapin
NCBI Synonym Full Names:SNAP associated protein
NCBI Official Symbol:SNAPIN��
NCBI Official Synonym Symbols:BLOS7; BORCS3; SNAPAP; BLOC1S7��
NCBI Protein Information:SNARE-associated protein Snapin
UniProt Protein Name:SNARE-associated protein Snapin
UniProt Synonym Protein Names:Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; Synaptosomal-associated protein 25-binding protein; SNAP-associated protein
Protein Family:SNAPIN protein
UniProt Gene Name:SNAPIN��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95295
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