Enzymes Recombinant Proteins

Human SMUG1 Recombinant Protein (RPPB2277)

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RPPB2277

Description

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Product Name:Human SMUG1 Recombinant Protein
Product Code:RPPB2277
Size:20µg
Species:Human
Target:SMUG1
Synonyms:Single-strand selective monofunctional uracil DNA glycosylase, SMUG1, FDG, UNG3, HMUDG.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SMUG1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMPQAFLL GSIHEPAGAL MEPQPCPGSL AESFLEEELR LNAELSQLQF SEPVGIIYNP VEYAWEPHRN YVTRYCQGPK EVLFLGMNPG PFGMAQTGVP FGEVSMVRDW LGIVGPVLTP PQEHPKRPVL GLECPQSEVS GARFWGFFRN LCGQPEVFFH HCFVHNLCPL LFLAPSGRNL TPAELPAKQR EQLLGICDAA LCRQVQLLGV RLVVGVGRLA EQRARRALAG LMPEVQVEGL LHPSPRNPQA NKGWEAVAKE RLNELGLLPL LLK

Single-strand-selective monofunctional uracil-DNA glycosylase (SMUG1) is an enzyme responsible for recognizing base lesions in the genome and initiating base excision DNA repair. SMUG1 participates in base excision repair by removing uracil from single- and double-stranded DNA. SMUG1 serves as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has inclination for single-stranded DNA substrates. SMUG1 activity is greater against mismatches (U/G) than against matches (U/A).

SMUG1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 293 amino acids (1-270) and having a molecular mass of 32.3kDa.SMUG1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SMUG1: Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.2.2.-; Hydrolase; DNA repair, damage

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: nucleolus; nucleoplasm

Molecular Function:DNA N-glycosylase activity; oxidized pyrimidine base lesion DNA N-glycosylase activity; protein binding; single-strand selective uracil DNA N-glycosylase activity; uracil DNA N-glycosylase activity

Biological Process: base-excision repair; depyrimidination

NCBI Summary:This gene encodes a protein that participates in base excision repair by removing uracil from single- and double-stranded DNA. Many alternatively spliced transcript variants exist for this gene; the full-length nature is known for some but not all of the variants. [provided by RefSeq, Aug 2011]
UniProt Code:Q53HV7
NCBI GenInfo Identifier:116242793
NCBI Gene ID:23583
NCBI Accession:Q53HV7.2
UniProt Secondary Accession:Q53HV7,O95862, Q0D2M0, Q8NB71, Q9BWC8, A8K2K9,
UniProt Related Accession:Q53HV7
Molecular Weight:19,627 Da
NCBI Full Name:Single-strand selective monofunctional uracil DNA glycosylase
NCBI Synonym Full Names:single-strand-selective monofunctional uracil-DNA glycosylase 1
NCBI Official Symbol:SMUG1��
NCBI Official Synonym Symbols:FDG; UNG3; HMUDG��
NCBI Protein Information:single-strand selective monofunctional uracil DNA glycosylase
UniProt Protein Name:Single-strand selective monofunctional uracil DNA glycosylase
Protein Family:Single-strand selective monofunctional uracil DNA glycosylase
UniProt Gene Name:SMUG1��
UniProt Entry Name:SMUG1_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q53HV7
Research Area:
Enzymes
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