Epigenetics & Nuclear Signaling Recombinant Proteins
Human SMAD4 Recombinant Protein (RPES5146)
- SKU:
- RPES5146
Description
Human SMAD4 Recombinant Protein
SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
| Product Name: | Human SMAD4 Recombinant Protein (RPES5146) |
| Product Code: | RPES5146 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Mothers Against Decapentaplegic Homolog 4, MAD Homolog 4, Mothers Against DPP Homolog 4, Deletion Target in Pancreatic Carcinoma 4, SMAD Family Member 4, SMAD 4, Smad4, hSMAD4, SMAD4, DPC4, MADH4 |
| Accession: | Q13485 |
| Sequence: | Met 1-Asp552 |
| Fusion tag: | C-6His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Mothers Against Decapentaplegic Homolog 4 is produced by our E.coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 85 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 61.5 kDa |
| AP Mol Mass: | 62 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM TrisHCl?pH8.0. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Epigenetics & Nuclear Signaling |
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