Human SMAC/DIABLO Recombinant Protein (RPPB4713)

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RPPB4713

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Product Name:Human SMAC/DIABLO Recombinant Protein
Product Code:RPPB4713
Size:20µg
Species:Human
Target:SMAC/DIABLO
Synonyms:Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein, Direct IAP-binding protein with low pI, DIABLO, SMAC, SMAC3, DIABLO-S, FLJ10537, FLJ25049.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The Smac/Diablo solution contains 20mM Tris pH-7.5.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MASMTGGQQM GRGSMAVPIA QKSEPHSLSS EALMRRAVSL VTDSTSTFLS QTTYALIEAI TEYTKAVYTL TSLYRQYTSL LGKMNSEEED EVWQVIIGAR AEMTSKHQEY LKLETTWMTA VGLSEMAAEA AYQTGADQAS ITARNHIQLV KLQVEEVHQL SRKAETKLAE AQIEELRQKT QEEGEERAES EQEAYLRED

Smac/Diablo is a proapoptotic protein that increases caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by its binding to the inhibitor of apoptosis proteins (IAPs) and removing their inhibitory activity. Smac/Diablo is a mitochondrial protein which enters the cytosol when cells go through apoptosis, and it moderates the caspase inhibition of IAPs.Smac/DIABLO expression is associated with the result of renal cell carcinoma.Dimeric form of Smac/DIABLO implies that once expressed in the cell the protein has a little probability of dissociation and, thus, loss of function. Survivin, Smac/DIABLO, & PKC-A play an important part in the inhibition of apoptosis by FGF-2 in human small cell lung cancer cells. Mitochondrial survivin associates with Smac/DIABLO, delaying its release. Decreased expression of Smac protein takes part in ovarian carcinogenesis and chemotherapeutic resistance. Smac/DIABLO plays a role in tumor cells during the pathway of apoptosis induction. SMAC protein is regulated by XIAP and degraded by proteasome. SMAC protein takes part inleukemic cell apoptosis. Smac is released during stress-induced apoptosis in multiple myeloma cells.

Smac/Diablo Human Recombinant fused to N-terminal T7-Tag produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22 kDa.

UniProt Protein Function:DIABLO: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol

Molecular Function:protein binding

Biological Process: caspase activation; neuron apoptosis; induction of apoptosis via death domain receptors; apoptosis; positive regulation of apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress

Disease: Deafness, Autosomal Dominant 64

NCBI Summary:This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
UniProt Code:Q9NR28
NCBI GenInfo Identifier:18203316
NCBI Gene ID:56616
NCBI Accession:Q9NR28.1
UniProt Secondary Accession:Q9NR28,Q6W3F3, Q96LV0, Q9BT11, Q9HAV6, B2RDQ0,
UniProt Related Accession:Q9NR28
Molecular Weight:239
NCBI Full Name:Diablo homolog, mitochondrial
NCBI Synonym Full Names:diablo, IAP-binding mitochondrial protein
NCBI Official Symbol:DIABLO��
NCBI Official Synonym Symbols:SMAC; DFNA64��
NCBI Protein Information:diablo homolog, mitochondrial; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase
UniProt Protein Name:Diablo homolog, mitochondrial
UniProt Synonym Protein Names:Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
Protein Family:Diablo
UniProt Gene Name:DIABLO��
UniProt Entry Name:DBLOH_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NR28
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