Human SIRT5 Recombinant Protein (RPPB4694)
- SKU:
- RPPB4694
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NXA8
Description
| Product Name: | Human SIRT5 Recombinant Protein |
| Product Code: | RPPB4694 |
| Size: | 20µg |
| Species: | Human |
| Target: | SIRT5 |
| Synonyms: | Sirtuin 5, NAD-Dependent Lysine Demalonylase AndDesuccinylase Sirtuin-5,Mitochondria, NAD-Dependent Protein Deacylase Sirtuin-5Mitochondrial, Silent Mating Type Information Regulation 2 (S.Cerevisiae)Homolog 5, NAD-Dependent Deacetylase Sirtuin-5, Regulatory Protein SIR2 Homolog5, SIR2-Like Protein 5, SIR2L5, Sirtuin Type 5, Sir2-Like 5, EC 3.5.1.-. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | SIRT5 protein solution (0.5mg/ml) containing 20mMTris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSARPSSSM ADFRKFFAKA KHIVIISGAG VSAESGVPTF RGAGGYWRKWQAQDLATPLA FAHNPSRVWE FYHYRREVMG SKEPNAGHRA IAECETRLGK QGRRVVVITQ NIDELHRKAGTKNLLEIHGS LFKTRCTSCG VVAENYKSPI CPALSGKGAP EPGTQDASIP VEKLPRCEEA GCGGLLRPHVVWFGENLDPA ILEEVDRELA HCDLCLVVGT SSVVYPAAMF APQVAARGVP VAEFNTETTP ATNRFRFHFQGPCGTTLPEA LACHENETVS |
SIRT5 belongs to the Sirtuin family of proteins, homologs to the yeast Sir2 protein. Sirtuin family members hold a sirtuin core domain and grouped into four classes. The protein encoded by this gene is included in class III of the sirtuin family. Human sirtuins functions were not yet determined; still, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies propose that human sirtuin could operate as intracellular regulatory protein with mono-ADP-ribosyltransferase activity.�
SIRT5 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain topological domain containing 300 aminoacids (34-310 a.a) and having a molecular mass of 32.5kDa. SIRT5 is fused to a 23amino acid His-tag at N-terminus & purified by proprietary chromatographictechniques.
| UniProt Protein Function: | SIRT5: NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Has weak NAD- dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro. Belongs to the sirtuin family. Class III subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; EC 3.5.1.-; Deacetylase Chromosomal Location of Human Ortholog: 6p23 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial intermembrane space; cytosol; nucleus Molecular Function:zinc ion binding; NAD+ ADP-ribosyltransferase activity Biological Process: protein amino acid ADP-ribosylation; protein amino acid deacetylation; chromatin silencing |
| NCBI Summary: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class III of the sirtuin family. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q9NXA8 |
| NCBI GenInfo Identifier: | 38258652 |
| NCBI Gene ID: | 23408 |
| NCBI Accession: | Q9NXA8.2 |
| UniProt Secondary Accession: | Q9NXA8,Q5T294, Q5T295, Q9Y6E6, B4DFM4, B4DYJ5, F5H5Z9 |
| UniProt Related Accession: | Q9NXA8 |
| Molecular Weight: | 310 |
| NCBI Full Name: | NAD-dependent protein deacylase sirtuin-5, mitochondrial |
| NCBI Synonym Full Names: | sirtuin 5 |
| NCBI Official Symbol: | SIRT5�� |
| NCBI Official Synonym Symbols: | SIR2L5�� |
| NCBI Protein Information: | NAD-dependent protein deacylase sirtuin-5, mitochondrial; SIRT5; sir2-like 5; sirtuin type 5; NAD-dependent deacetylase sirtuin-5; silent mating type information regulation 2, S.cerevisiae, homolog 5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial |
| UniProt Protein Name: | NAD-dependent protein deacylase sirtuin-5, mitochondrial |
| UniProt Synonym Protein Names: | Regulatory protein SIR2 homolog 5; SIR2-like protein 5 |
| Protein Family: | NAD-dependent protein deacylase sirtuin |
| UniProt Gene Name: | SIRT5�� |
| UniProt Entry Name: | SIR5_HUMAN |
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