Human SH2D1B Recombinant Protein (RPPB4671)

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SKU:
RPPB4671
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O14796

Description

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Product Name:Human SH2D1B Recombinant Protein
Product Code:RPPB4671
Size:10µg
Species:Human
Target:SH2D1B
Synonyms:SH2 Domain Containing 1B, EWS/FLI1-Activated Transcript 2, EAT-2, EAT2, SH2 Domain-Containing Molecule EAT2, SH2 Domain-Containing Protein 1B, SH2 domain-containing protein 1B, EWS/FLI1-activated transcript 2, EAT-2.
Source:Sf9 Insect cells
Physical Appearance:Sterile filtered colorless solution.
Formulation:SH2D1B protein solution (0.25mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:ADPMDLPYYH GRLTKQDCET LLLKEGVDGN FLLRDSESIP GVLCLCVSFK NIVYTYRIFR EKHGYYRIQT AEGSPKQVFP SLKELISKFE KPNQGMVVHL LKPIKRTSPS LRWRGLKLEL ETFVNSNSDY VDVLPHHHHH H

SH2 domain-containing protein 1B, also known as SH2D1B activates NK cells. SH2D1B participates in controlling signal transduction through at least 4 receptors, CD84, SLAMF1, LY9 and CD244, expressed on the surface of professional antigen-presenting cells. Two isoforms of the human SH2D1B are produced by alternative splicing. Superior Mesenteric Artery Syndrome is one of the diseases which is associated with SH2D1B.�

SH2D1B Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 141 amino acids (1-132a.a.) and having a molecular mass of 16.4kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). SH2D1B is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Cytoplasmic adapter regulating receptors of the signaling lymphocytic activation molecule (SLAM) family such as CD84, SLAMF1, LY9 and CD244 (PubMed:11689425). In SLAM signaling seems to cooperate with SH2D1A/SAP. Plays a role in regulation of effector functions of natural killer (NK) cells by controlling signal transduction through CD244/2B4 without effecting its tyrosine phosphorylation; downstream signaling involves PLCG1 and ERK activation (PubMed:24687958). Activation of SLAMF7-mediated NK cell function does not effect receptor tyrosine phosphorylation but distal signaling. In the context of NK cell-mediated cytotoxicity does not enhance conjugate formation with target cells but stimulates polarization of the microtubule-organizing center and cytotoxic granules toward the NK cell synapse (PubMed:24687958). Negatively regulates CD40-induced cytokine production in dendritic cells downstream of SLAM family receptors probably by inducing activation of the PI3K pathway to inhibit p38 MAPK and JNK activation.
NCBI Summary:By binding phosphotyrosines through its free SRC (MIM 190090) homology-2 (SH2) domain, EAT2 regulates signal transduction through receptors expressed on the surface of antigen-presenting cells (Morra et al., 2001 [PubMed 11689425]).[supplied by OMIM, Mar 2008]
UniProt Code:O14796
NCBI GenInfo Identifier:23503051
NCBI Gene ID:117157
NCBI Accession:O14796.2
UniProt Secondary Accession:O14796,Q5T0L1, Q8NI18, Q969K9, B2RBN6,
UniProt Related Accession:O14796
Molecular Weight:9,024 Da
NCBI Full Name:SH2 domain-containing protein 1B
NCBI Synonym Full Names:SH2 domain containing 1B
NCBI Official Symbol:SH2D1B��
NCBI Official Synonym Symbols:EAT2��
NCBI Protein Information:SH2 domain-containing protein 1B
UniProt Protein Name:SH2 domain-containing protein 1B
UniProt Synonym Protein Names:EWS/FLI1-activated transcript 2; EAT-2
Protein Family:SH2 domain-containing protein
UniProt Gene Name:SH2D1B��
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