Human SF3B14 Recombinant Protein (RPPB4658)

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SKU:
RPPB4658
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y3B4

Description

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Product Name:Human SF3B14 Recombinant Protein
Product Code:RPPB4658
Size:10µg
Species:Human
Target:SF3B14
Synonyms:Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14, CGI-110, HSPC175, HT006, P14, SAP14, SF3B14a.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SF3B14 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and�30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK

SF3B14 is a 125 amino acid nuclear protein which is a component of the splicing factor 3b complex. Splicing factor 3b is involved with s with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. SF3B14 which is required for the splicing of pre-mRNA enters the spliceosome and connect with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex.

SF3B14 Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�145 amino acids (1-125a.a.) and having a molecular mass of 16.7kDa. The SF3B14 is purified by proprietary chromatographic techniques.

UniProt Protein Function:SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
UniProt Protein Details:

Protein type:Spliceosome; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 2p23

Cellular Component: nucleoplasm; snRNP U2; U12-dependent spliceosome

Molecular Function:nucleotide binding

Biological Process: blastocyst formation; gene expression; nuclear mRNA splicing, via spliceosome; RNA splicing

NCBI Summary:This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y3B4
NCBI GenInfo Identifier:7706326
NCBI Gene ID:51639
NCBI Accession:NP_057131.1
UniProt Secondary Accession:Q9Y3B4,Q53TM1,
UniProt Related Accession:Q9Y3B4
Molecular Weight:14,585 Da
NCBI Full Name:splicing factor 3B subunit 6
NCBI Synonym Full Names:splicing factor 3b subunit 6
NCBI Official Symbol:SF3B6��
NCBI Official Synonym Symbols:P14; Ht006; SAP14; SAP14a; SF3B14; CGI-110; HSPC175; SF3B14a��
NCBI Protein Information:splicing factor 3B subunit 6
UniProt Protein Name:Splicing factor 3B subunit 6
UniProt Synonym Protein Names:Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDa
Protein Family:Splicing factor
UniProt Gene Name:SF3B6��
UniProt Entry Name:SF3B6_HUMAN
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