Human SERPINH1 Recombinant Protein (RPPB5238)

(No reviews yet) Write a Review
SKU:
RPPB5238

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human SERPINH1 Recombinant Protein
Product Code:RPPB5238
Size:50µg
Species:Human
Target:SERPINH1
Synonyms:HSP47, HSP-47, Colligin-1, CBP1, Collagen Binding Protein-1, Serpin Peptidase Inhibitor Clade-H memebr 1, Serpin H1, Collagen-binding protein, Colligin, 47 kDa heat shock protein, Rheumatoid arthritis-related antigen RA-A47, Arsenic-transactivated protein 3, AsTP3, Cell proliferation-inducing gene 14 protein, SERPINH1, CBP2, gp46, PIG14, PPROM, RA-A47, SERPINH2.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The SERPINH1 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAAEVKKPAA AAAPGTAEKL SPKAATLAER SAGLAFSLYQ AMAKDQAVEN ILVSPVVVAS SLGLVSLGGK ATTASQAKAV LSAEQLRDEE VHAGLGELLR SLSNSTARNV TWKLGSRLYG PSSVSFADDF VRSSKQHYNC EHSKINFRDK RSALQSINEW AAQTTDGKLP EVTKDVERTD GALLVNAMFF KPHWDEKFHH KMVDNRGFMV TRSYTVGVMM MHRTGLYNYY DDEKEKLQIV EMPLAHKLSS LIILMPHHVE PLERLEKLLT KEQLKIWMGK MQKKAVAISL PKGVVEVTHD LQKHLAGLGL TEAIDKNKAD LSRMSGKKDL YLASVFHATA FELDTDGNPF DQDIYGREEL RSPKLFYADH PFIFLVRDTQ SGSLLFIGRL VRPKGDKMRD EL

SERPINH1 is part of the serpin superfamily of serine proteinase inhibitors. SEPINH1 expression is induced by heat shock. HSP47 localizes to the endoplasmic reticulum lumen and binds collagen therefore it is a molecular chaperone which takes part in the maturation of collagen molecules, facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Autoantibodies to HSP47 protein have been found in rheumatoid arthritis. SERPINH1 binds specifically to collagen and acts as a chaperone in the biosynthetic pathway of collagen.

Recombinant Human HSP47 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 422 amino acids (18-418 a.a) and having a molecular mass of 46.8kDa. HSP47 human recombinant is fused to a 20 amino acid His Tag at N-terminus and purified by convential chromatogrpahy techniques.

UniProt Protein Function:SERPINH1: Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. Defects in SERPINH1 are the cause of osteogenesis imperfecta type 10 (OI10). A connective tissue disorder characterized by bone fragility, low bone mass, bowing of limbs due to multiple fractures, short limb dwarfism and blue sclerae. Belongs to the serpin family.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 11q13.5

Cellular Component: extracellular space; endoplasmic reticulum; endoplasmic reticulum lumen; ER-Golgi intermediate compartment

Molecular Function:serine-type endopeptidase inhibitor activity; collagen binding; unfolded protein binding

Biological Process: extracellular matrix organization and biogenesis; collagen fibril organization; protein maturation; collagen biosynthetic process; response to unfolded protein

Disease: Osteogenesis Imperfecta, Type X; Preterm Premature Rupture Of The Membranes

NCBI Summary:This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
UniProt Code:P50454
NCBI GenInfo Identifier:20141241
NCBI Gene ID:871
NCBI Accession:P50454.2
UniProt Secondary Accession:P50454,P29043, Q5XPB4, Q6NSJ6, Q8IY96, Q9NP88, B3KVJ3
UniProt Related Accession:P50454
Molecular Weight:418
NCBI Full Name:Serpin H1
NCBI Synonym Full Names:serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
NCBI Official Symbol:SERPINH1��
NCBI Official Synonym Symbols:CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2��
NCBI Protein Information:serpin H1; colligin-1; colligin-2; 47 kDa heat shock protein; arsenic-transactivated protein 3; rheumatoid arthritis antigen A-47; cell proliferation-inducing gene 14 protein; rheumatoid arthritis-related antigen RA-A47; serine (or cysteine) proteinase in
UniProt Protein Name:Serpin H1
UniProt Synonym Protein Names:47 kDa heat shock protein; Arsenic-transactivated protein 3; AsTP3; Cell proliferation-inducing gene 14 protein; Collagen-binding protein; Colligin; Rheumatoid arthritis-related antigen RA-A47
UniProt Gene Name:SERPINH1��
UniProt Entry Name:SERPH_HUMAN
View AllClose

Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P50454
View AllClose

Related Products