Enzymes Recombinant Proteins

Human SEPSECS Recombinant Protein (RPPB2268)

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SKU:
RPPB2268
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HD40
Research Area:
Enzymes

Description

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Product Name:Human SEPSECS Recombinant Protein
Product Code:RPPB2268
Size:20µg
Species:Human
Target:SEPSECS
Synonyms:SLA, SLA/LP autoantigen, SLA-p35, Selenocysteinyl-tRNA(Sec) synthase, Selenocysteine synthase, Liver-pancreas antigen, Soluble liver antigen, Sec synthase, UGA suppressor tRNA-associated protein.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The SEPSECS protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 100mM NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLLLEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVARRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEGDELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQEISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTVSGYTFR GFMSHTNNYP CAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERSKESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S

SEPSECS catalyzes the last step of sec synthesis by way of converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Furthermore, SEPSECS protein is considered a specific marker of autoimmune hepatitis.

SEPSECS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 521 amino acids (1-501a.a.) and having a molecular mass of 57.9kDa.SEPSECS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transferase; EC 2.9.1.2; RNA-binding

Chromosomal Location of Human Ortholog: 4p15.2

Cellular Component: cytoplasm; nucleus

Molecular Function:protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding

Biological Process: selenocysteine incorporation

Disease: Pontocerebellar Hypoplasia, Type 2d

NCBI Summary:The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
UniProt Code:Q9HD40
NCBI GenInfo Identifier:62287911
NCBI Gene ID:51091
NCBI Accession:Q9HD40.2
UniProt Secondary Accession:Q9HD40,Q0D2P3, Q17RT1, Q9NXZ5, Q9UGM9, Q9Y353, A8K8W1
UniProt Related Accession:Q9HD40
Molecular Weight:501
NCBI Full Name:O-phosphoseryl-tRNA(Sec) selenium transferase
NCBI Synonym Full Names:Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
NCBI Official Symbol:SEPSECS��
NCBI Official Synonym Symbols:LP; SLA; PCH2D; SLA/LP��
NCBI Protein Information:O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; soluble liver antigen/liver pancreas antigen
UniProt Protein Name:O-phosphoseryl-tRNA(Sec) selenium transferase
UniProt Synonym Protein Names:Liver-pancreas antigen; LP; SLA-p35; SLA/LP autoantigen; Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS; Soluble liver antigen; SLA; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein
Protein Family:O-phosphoseryl-tRNA(Sec) selenium transferase
UniProt Gene Name:SEPSECS��
UniProt Entry Name:SPCS_HUMAN
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