Developmental Biology Recombinant Proteins
Human Semaphorin 5A/SEMA5A Recombinant Protein (RPES4354)
- SKU:
- RPES4354
Description
Human Semaphorin 5A/SEMA5A Recombinant Protein
Semaphorin-5A (SEMA5A) is a member of the Semaphorin family of axon guidance molecules. SEMA5A is a 140 kDa protein. Class 5 Semaphorins are type I transmembrane glycoproteins with an N- terminal Sema domain and multiple juxtamembrane type 1 Thrombospondin (TSP) repeats within their extracellular domains. SEMA5A is expressed in neuroepithelial cells surrounding retinal axons, oligodendrocytes, the base of limb buds, the mesoderm surrounding cranial vessels , and the cardiac atrial septum and endocardial cushions, Human SEMA5A cDNA encodes a signal sequence, a extracellular domain (ECD), a transmembrane sequence and an cytoplasmic portion. SEMA5A mutations have been implicated in the genetic syndrome,cri-du-chat,while some polymorphisms may increase risk for neurodegenerative diseases such as Parkinson. The expression of SEMA5A may be upregulated in metastatic cancer cells and downregulated in autism.
| Product Name: | Human Semaphorin 5A/SEMA5A Recombinant Protein (RPES4354) |
| Product Code: | RPES4354 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | Semaphorin-5A, Semaphorin-F, Sema F, SEMA5A, SEMAF |
| Accession: | Q13591 |
| Sequence: | Glu23-Thr765 |
| Fusion tag: | C-6His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Semaphorin 5A is produced by our Mammalian expression system and the target gene encoding Glu23-Thr765 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 84.7 kDa |
| AP Mol Mass: | 100 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Developmental Biology |
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