Human SCCA2/Serpin B4 PharmaGenie ELISA Kit (SBRS1058)
(No reviews yet)
Write a Review
- SKU:
- SBRS1058
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Human SCCA2/Serpin B4 PharmaGenie ELISA Kit (SBRS1058)
| Product SKU: | SBRS1058 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase/ADA, AIF, AKR1C4 , ASAH2, BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR/CD299, eNOS, ENPP2/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase/GUSB, Inhibin A, LILRB4/CD85k/ILT3, Neuroglycan C/CSPG5, NKp46/NCR1, Neuronal Pentraxin R/NPTXR, ROR2, Siglec-2/CD22, SIRP alpha/CD172a, SorCS1, Trypsin 1/PRSS1, Trypsin 3/PRSS3. |
| Uniprot: | P48594 |
| Gene ID: | 6318 |
| Gene Names: | PI11 / SCCA2 / SERPINB4 |
| Synonyms: | Serpin B4 (Leupin) (Peptidase inhibitor 11) (PI-11) (Squamous cell carcinoma antigen 2) (SCCA-2) |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 0.23 ng/ml - 50 ng/ml |
| Sensitivity: | 0.23 ng/ml |
| Recommended Dilution: | 3 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human SCCA2/Serpin B4 PharmaGenie ELISA Kit (SBRS1058) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
Related Products
Human Serpin F1 PharmaGenie ELISA Kit (SBRS1079)
system_update_altDatasheetHuman Serpin F1 PharmaGenie ELISA Kit (SBRS1079)Overview
Human Serpin G1 PharmaGenie ELISA Kit (SBRS1081)
system_update_altDatasheetHuman Serpin G1 PharmaGenie ELISA Kit (SBRS1081)Overview
Human Serpin A1 PharmaGenie ELISA Kit (SBRS1072)
system_update_altDatasheetHuman Serpin A1 PharmaGenie ELISA Kit (SBRS1072)Overview
Human Serpin B6 PharmaGenie ELISA Kit (SBRS1077)
system_update_altDatasheetHuman Serpin B6 PharmaGenie ELISA Kit (SBRS1077)The Assay Genie Human Serpin B6 ELISA Kit will require other equipment and materials to carry out the assay...