Enzymes Recombinant Proteins

Human SARS Recombinant Protein (RPPB2262)

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SKU:
RPPB2262
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P49591
Research Area:
Enzymes

Description

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Product Name:Human SARS Recombinant Protein
Product Code:RPPB2262
Size:10µg
Species:Human
Target:SARS
Synonyms:Serine--tRNA ligase cytoplasmic, Seryl-tRNA synthetase, SerRS, Seryl-tRNA(Ser/Sec) synthetase, SARS, SERS.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The SARS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 100mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMVLDLDL FRVDKGGDPA LIRETQEKRF KDPGLVDQLV KADSEWRRCR FRADNLNKLK NLCSKTIGEK MKKKEPVGDD ESVPENVLSF DDLTADALAN LKVSQIKKVR LLIDEAILKC DAERIKLEAE RFENLREIGN LLHPSVPISN DEDVDNKVER IWGDCTVRKK YSHVDLVVMV DGFEGEKGAV VAGSRGYFLK GVLVFLEQAL IQYALRTLGS RGYIPIYTPF FMRKEVMQEV AQLSQFDEEL YKVIGKGSEK SDDNSYDEKY LIATSEQPIA ALHRDEWLRP EDLPIKYAGL STCFRQEVGS HGRDTRGIFR VHQFEKIEQF VYSSPHDNKS WEMFEEMITT AEEFYQSLGI PYHIVNIVSG SLNHAASKKL DLEAWFPGSG AFRELVSCSN CTDYQARRLR IRYGQTKKMM DKVEFVHMLN ATMCATTRTI CAILENYQTE KGITVPEKLK EFMPPGLQEL IPFVKPAPIE QEPSKKQKKQ HEGSKKKAAA RDVTLENRLQ NMEVTDA

Seryl-tRNA synthetase, cytoplasmic (SARS) is a member of the class-II aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetases� role is to catalyze the aminoacylation of tRNAs by their corresponding amino acids, as a result linking amino acids with tRNA-contained nucleotide triplets. The SARS enzyme catalyzes the attachment of serine to tRNA (Ser). SARS enzyme is probably able to aminoacylate tRNA (Sec) with serine, to form the misacylated tRNA L-seryl-tRNA (Sec), which will be then converted into selenocysteinyl-tRNA (Sec).

SARS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 537 amino acids (1-514) and having a molecular mass of 61.2kDa.SARS is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SARS: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
UniProt Protein Details:

Protein type:Translation; EC 6.1.1.11; RNA processing; Ligase

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: cytoplasm; cytosol

Molecular Function:RNA binding; serine-tRNA ligase activity

Biological Process: selenocysteine metabolic process; seryl-tRNA aminoacylation; translation; tRNA aminoacylation for protein translation; tRNA processing

NCBI Summary:This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown. [provided by RefSeq, Jul 2010]
UniProt Code:P49591
NCBI GenInfo Identifier:19860217
NCBI Gene ID:6301
NCBI Accession:P49591.3
UniProt Secondary Accession:P49591,Q5T5C8, Q9NSE3, B2R6Y9,
UniProt Related Accession:P49591
Molecular Weight:58,777 Da
NCBI Full Name:Serine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:seryl-tRNA synthetase
NCBI Official Symbol:SARS��
NCBI Official Synonym Symbols:SERS; SERRS��
NCBI Protein Information:serine--tRNA ligase, cytoplasmic
UniProt Protein Name:Serine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Seryl-tRNA synthetase; SerRS; Seryl-tRNA(Ser/Sec) synthetase
Protein Family:Serine--tRNA ligase
UniProt Gene Name:SARS��
UniProt Entry Name:SYSC_HUMAN
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