Human RTK Phosphorylation Array (71 targets) (SARB0064)

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SKU:
SARB0064
Product Type:
Phosphorylation Array
Analytes:
ABL1
Analytes:
ACK
Analytes:
ALK-1
Analytes:
Axl
Analytes:
Blk
Analytes:
BMX
Analytes:
Btk
Analytes:
Csk
Analytes:
Dtk
Analytes:
EGFR
Analytes:
EphA1
Analytes:
EphA2
Analytes:
EphA3
Analytes:
EphA4
Analytes:
EphA5
Analytes:
EphA6
Analytes:
EphA7
Analytes:
EphA8
Analytes:
EphB1
Analytes:
EphB2
Analytes:
EphB3
Analytes:
EphB4
Analytes:
EphB6
Analytes:
ErbB2
Analytes:
ErbB3
Analytes:
ErbB4
Analytes:
FAK
Analytes:
FER
Analytes:
FGFR1
Analytes:
FGFR2
Analytes:
FGFR2 alpha isoform
Analytes:
Fgr
Analytes:
FRK
Analytes:
Fyn
Analytes:
Hck
Analytes:
HGFR
Analytes:
IGF-1 R
Analytes:
Insulin R (CD220)
Analytes:
Itk
Analytes:
JAK1
Analytes:
JAK2
Analytes:
JAK3
Analytes:
LCK
Analytes:
LTK
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0064
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:71
Gene Symbols:ABL1, AXL, BLK, BMX, BTK, CSF1R, CSK, DDR2, EGFR, EPHA1|EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHB1, EPHB2, EPHB3, EPHB4, EPHB6, ERBB2, ERBB3, ERBB4, FER, FGFR1, FGFR2, FGR, FLT4, FRK, FYN, HCK, IGF1R, INSR, ITK, JAK1, JAK2, JAK3, KDR, KIT, LCK, LTK, LYN, MATK, MET, MUSK, NGFR, NTRK2, PDGFRA, PDGFRB, PTK2, PTK2B, RET, ROR1, ROR2, ROS1, RYK, SLPI, SRMS, SYK, TEC, TEK, TIE1, TNK1, TNK2, TXK, TYK2, TYRO3, ZAP70
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:PI3K-AKT Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
ABL1
  • Gene Symbol: ABL1
  • Gene ID: 25
  • Accession #: P00519
ACK
  • Gene Symbol: TNK2
  • Gene ID: 10188
  • Accession #: Q07912
ALK-1
  • Gene Symbol: ALK
  • Gene ID: 238
  • Accession #: Q9UM73
Axl
  • Gene Symbol: Axl
  • Gene ID: 558
  • Accession #: P30530
Blk
  • Gene Symbol: BLK
  • Gene ID: 640
  • Accession #: P51451
BMX
  • Gene Symbol: BMX
  • Gene ID: 660
  • Accession #: P51813
Btk
  • Gene Symbol: BTK
  • Gene ID: 695
  • Accession #: Q06187
Csk
  • Gene Symbol: CSK
  • Gene ID: 1445
  • Accession #: P41240
Dtk
  • Gene Symbol: TYRO3
  • Gene ID: 7301
  • Accession #: Q06418
EGFR
  • Gene Symbol: EGFR
  • Gene ID: 1956
  • Accession #: P00533
EphA1
  • Gene Symbol: EphA1
  • Gene ID: 2041
  • Accession #: P21709
EphA2
  • Gene Symbol: EphA2
  • Gene ID: 1969
  • Accession #: P29317
EphA3
  • Gene Symbol: EphA3
  • Gene ID: 2042
  • Accession #: P29320
EphA4
  • Gene Symbol: EphA4
  • Gene ID: 2043
  • Accession #: P54764
EphA5
  • Gene Symbol: EphA5
  • Gene ID: 2044
  • Accession #: P54756
EphA6
  • Gene Symbol: EphA6
  • Gene ID: 285220
  • Accession #: Q9UF33
EphA7
  • Gene Symbol: EphA7
  • Gene ID: 2045
  • Accession #: Q15375
EphA8
  • Gene Symbol: EphA8
  • Gene ID: 2046
  • Accession #: P29322
EphB1
  • Gene Symbol: EphB1
  • Gene ID: 2047
  • Accession #: P54762
EphB2
  • Gene Symbol: EphB2
  • Gene ID: 2048
  • Accession #: P29323
EphB3
  • Gene Symbol: EphB3
  • Gene ID: 2049
  • Accession #: P54753
EphB4
  • Gene Symbol: EphB4
  • Gene ID: 2050
  • Accession #: P54760
EphB6
  • Gene Symbol: EphB6
  • Gene ID: 2051
  • Accession #: O15197
ErbB2
  • Gene Symbol: ErbB2
  • Gene ID: 2064
  • Accession #: P04626
ErbB3
  • Gene Symbol: ErbB3
  • Gene ID: 2065
  • Accession #: P21860
ErbB4
  • Gene Symbol: ErbB4
  • Gene ID: 2066
  • Accession #: Q15303
FAK
  • Gene Symbol: PTK2
  • Gene ID: 5747
  • Accession #: Q05397
FER
  • Gene Symbol: FER
  • Gene ID: 2241
  • Accession #: P16591
FGFR1
  • Gene Symbol: FGFR1
  • Gene ID: 2260
  • Accession #: P11362
FGFR2
  • Gene Symbol: FGFR2
  • Gene ID: 2263
  • Accession #: P21802
FGFR2 alpha isoform
  • Gene Symbol: FGFR2
  • Gene ID: 2263
  • Accession #: P21802
Fgr
  • Gene Symbol: FGR
  • Gene ID: 2268
  • Accession #: P09769
FRK
  • Gene Symbol: FRK
  • Gene ID: 2444
  • Accession #: P42685
Fyn
  • Gene Symbol: FYN
  • Gene ID: 2534
  • Accession #: P06241
Hck
  • Gene Symbol: HCK
  • Gene ID: 3055
  • Accession #: P08631
HGFR
  • Gene Symbol: MET
  • Gene ID: 4233
  • Accession #: P08581
IGF-1 R
  • Gene Symbol: IGF1R
  • Gene ID: 3480
  • Accession #: P08069
Insulin R (CD220)
  • Gene Symbol: INSR
  • Gene ID: 3643
  • Accession #: P06213
Itk
  • Gene Symbol: ITK
  • Gene ID: 3702
  • Accession #: Q08881
JAK1
  • Gene Symbol: JAK1
  • Gene ID: 3716
  • Accession #: P23458
JAK2
  • Gene Symbol: JAK2
  • Gene ID: 3717
  • Accession #: O60674
JAK3
  • Gene Symbol: JAK3
  • Gene ID: 3718
  • Accession #: P52333
LCK
  • Gene Symbol: LCK
  • Gene ID: 3932
  • Accession #: P06239
LTK
  • Gene Symbol: LTK
  • Gene ID: 4058
  • Accession #: P29376
Lyn
  • Gene Symbol: LYN
  • Gene ID: 4067
  • Accession #: P07948
MATK
  • Gene Symbol: MATK
  • Gene ID: 4145
  • Accession #: P42679
M-CSF R
  • Gene Symbol: CSF1R
  • Gene ID: 1436
  • Accession #: P07333
MUSK
  • Gene Symbol: MuSK
  • Gene ID: 4593
  • Accession #: O15146
NGFR (TNFRSF16)
  • Gene Symbol: NGFR
  • Gene ID: 4804
  • Accession #: P08138
PDGFRA
  • Gene Symbol: PDGFRA
  • Gene ID: 5156
  • Accession #: P16234
PDGFRB
  • Gene Symbol: PDGFRB
  • Gene ID: 5159
  • Accession #: P09619
PYK2
  • Gene Symbol: PTK2B
  • Gene ID: 2185
  • Accession #: Q14289
RET
  • Gene Symbol: RET
  • Gene ID: 5979
  • Accession #: P07949
ROR1
  • Gene Symbol: ROR1
  • Gene ID: 4919
  • Accession #: Q01973
ROR2
  • Gene Symbol: ROR2
  • Gene ID: 4920
  • Accession #: Q01974
ROS
  • Gene Symbol: ROS1
  • Gene ID: 6098
  • Accession #: P08922
RYK
  • Gene Symbol: RYK
  • Gene ID: 6259
  • Accession #: P34925
SCF R (CD117/c-kit)
  • Gene Symbol: KIT
  • Gene ID: 3815
  • Accession #: P10721
SRMS
  • Gene Symbol: SRMS
  • Gene ID: 6725
  • Accession #: Q9H3Y6
SYK
  • Gene Symbol: SYK
  • Gene ID: 6850
  • Accession #: P43405
Tec
  • Gene Symbol: TEC
  • Gene ID: 7006
  • Accession #: P42680
Tie-1
  • Gene Symbol: TIE1
  • Gene ID: 7075
  • Accession #: P35590
Tie-2
  • Gene Symbol: TEK
  • Gene ID: 7010
  • Accession #: Q02763
TNK1
  • Gene Symbol: TNK1
  • Gene ID: 8711
  • Accession #: Q13470
TRKB
  • Gene Symbol: NTRK2
  • Gene ID: 4915
  • Accession #: Q16620
TXK
  • Gene Symbol: TXK
  • Gene ID: 7294
  • Accession #: P42681
Tyk2
  • Gene Symbol: TYK2
  • Gene ID: 7297
  • Accession #: P29597
TYRO10 (DDR2/TKT)
  • Gene Symbol: DDR2
  • Gene ID: 4921
  • Accession #: Q16832
VEGFR2
  • Gene Symbol: KDR
  • Gene ID: 3791
  • Accession #: P35968
VEGFR3
  • Gene Symbol: FLT4
  • Gene ID: 2324
  • Accession #: P35916
ZAP70
  • Gene Symbol: ZAP70
  • Gene ID: 7535
  • Accession #: P43403
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human RTK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Anti-Phospho Tyrosine Detection Antibody
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer (1 x 5 ml)
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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