Human RSPO3 Recombinant Protein (RPPB4531)
- SKU:
- RPPB4531
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BXY4
Description
| Product Name: | Human RSPO3 Recombinant Protein |
| Product Code: | RPPB4531 |
| Size: | 20µg |
| Species: | Human |
| Target: | RSPO3 |
| Synonyms: | R-spondin-3, Protein with TSP type-1 repeat, hPWTSR, Roof plate-specific spondin-3, hRspo3, Thrombospondin type-1 domain-containing protein 2, RSPO3, PWTSR, THSD2, THSD2, CRISTIN1. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | RSPO3 was lyophilized from a 0.2�m filtered solution in 20mM PB, and 150mM NaCl pH-7.2. |
| Solubility: | It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 �g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO3 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS PAGE. |
| Amino Acid Sequence: | QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP SGYYGTRYPD INKCTKCKAD CDTCFNKNFC TKCKSGFYLH LGKCLDNCPE GLEANNHTME CVSIVHCEVS EWNPWSPCTK KGKTCGFKRG TETRVREIIQ HPSAKGNLCP PTNETRKCTV�DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS�HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA�LPAPIEKTIS KAKGQPREPQ VYTLPPSREE MTKNQVSLTC LVKGFYPSDI AVEWESNGQP�ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK�HHHHHH�� |
R-spondin-3 (RSPO3) belongs to the thrombospondin type 1 repeat supergene family. RSPO3 is a secreted protein which is widely expressed in many tissues. RSPO3 contains 2 Furin-like repeats which have been found in various eukaryotic proteins involved in the mechanism of signal transduction by receptor tyrosine kinases, and one TSP type-1 domain. RSPO3 acts as an activator of the beta-catenin signaling cascade, initiating TCF-dependent gene activation.
Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid Gln at position 22 to amino acid Val at position 201, fused to an FC, 6 x His-tag at C-terminus, containing a total of 498 amino acids and having a Mw of 47.9 kDa. The protein migrates at 61kDa on SDS-PAGE.�RSPO3 is a truncated protein that lacks amino acid�Gln at position 201 to amino acid H at position 272 and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | R-spondin-3: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Belongs to the R-spondin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 6q22.33 Cellular Component: extracellular space; extracellular region Molecular Function:heparin binding; G-protein-coupled receptor binding; frizzled binding; receptor binding Biological Process: Wnt receptor signaling pathway through beta-catenin |
| NCBI Summary: | This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9BXY4 |
| NCBI GenInfo Identifier: | 74752442 |
| NCBI Gene ID: | 84870 |
| NCBI Accession: | Q9BXY4.1 |
| UniProt Secondary Accession: | Q9BXY4,Q5VTV4, Q96K87, B2RC27, |
| UniProt Related Accession: | Q9BXY4 |
| Molecular Weight: | 33,233 Da |
| NCBI Full Name: | R-spondin-3 |
| NCBI Synonym Full Names: | R-spondin 3 |
| NCBI Official Symbol: | RSPO3�� |
| NCBI Official Synonym Symbols: | PWTSR; THSD2; CRISTIN1�� |
| NCBI Protein Information: | R-spondin-3; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2 |
| UniProt Protein Name: | R-spondin-3 |
| UniProt Synonym Protein Names: | Protein with TSP type-1 repeat; hPWTSR; Roof plate-specific spondin-3; hRspo3; Thrombospondin type-1 domain-containing protein 2 |
| Protein Family: | R-spondin |
| UniProt Gene Name: | RSPO3�� |
| UniProt Entry Name: | RSPO3_HUMAN |
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