Human RPL35A Recombinant Protein (RPPB4500)

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RPPB4500

Description

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Product Name:Human RPL35A Recombinant Protein
Product Code:RPPB4500
Size:20µg
Species:Human
Target:RPL35A
Synonyms:Ribosomal Protein L35a, Cell Growth-Inhibiting Gene 33 Protein, 60S Ribosomal Protein L35a, DBA5, L35A.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The RPL35A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSGRLWS KAIFAGYKRG LRNQREHTAL LKIEGVYARD ETEFYLGKRC AYVYKAKNNT VTPGGKPNKT RVIWGKVTRA HGNSGMVRAK FRSNLPAKAI GHRIRVMLYP SRI

Ribosomes, the organelles which catalyze protein synthesis, contain a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and nearly 80 structurally different proteins. RPL35A is a component of the 60S subunit of the ribosomal protein and a member of the L35AE family of ribosomal proteins. RPL35A is situated in the cytoplasm. The rat protein is known to bind to both initiator and elongator tRNAs, therefore, it is located at the P site, or P and A sites, of the ribosome. Though RPL35A was initially mapped to chromosome 18, it has been proven that it is located at 3q29-qter. Transcript variants utilizing alternative transcription initiation sites and alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are several processed pseudogenes of this gene spread all over the genome.

RPL35A Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 133 amino acids (1-110) and having a molecular mass of 14.9kDa.RPL35A is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site.
NCBI Summary:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015]
UniProt Code:P18077
NCBI GenInfo Identifier:16117791
NCBI Gene ID:6165
NCBI Accession:NP_000987.2
UniProt Secondary Accession:P18077,Q08ES9, Q9BVN7,
UniProt Related Accession:P18077
Molecular Weight:12
NCBI Full Name:60S ribosomal protein L35a
NCBI Synonym Full Names:ribosomal protein L35a
NCBI Official Symbol:RPL35A��
NCBI Official Synonym Symbols:DBA5; L35A; eL33��
NCBI Protein Information:60S ribosomal protein L35a
UniProt Protein Name:60S ribosomal protein L35a
UniProt Synonym Protein Names:Cell growth-inhibiting gene 33 protein; Large ribosomal subunit protein eL33
UniProt Gene Name:RPL35A��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P18077
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