Human RNF181 Recombinant Protein (RPPB4477)

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RPPB4477

Description

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Product Name:Human RNF181 Recombinant Protein
Product Code:RPPB4477
Size:20µg
Species:Human
Target:RNF181
Synonyms:Ring Finger Protein 181, E3 Ubiquitin-Protein Ligase RNF181, HSPC238, EC 6.3.2.-.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The RNF181 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI RGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT

E3 ubiquitin-protein ligase RNF181 (RNF181) accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the thioester form and subsequently directly transfers the ubiquitin to targeted substrates. RNF181 is widely expressed, with the highest levels in the liver and heart and the lowest levels in the brain and skeletal muscle. RNF181 is also expressed in platelets (at protein level).

RNF181 Human Recombinant produced in E. coli is a single polypeptide chain containing 176 amino acids (1-153) and having a molecular mass of 20.3 kDa.RNF181 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RNF181: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RNF181 family.
UniProt Protein Details:

Protein type:Ligase; Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2p11.2

Molecular Function:zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein autoubiquitination

NCBI Summary:RNF181 binds the integrin alpha-IIb (ITGA2B; MIM 607759)/beta-3 (ITGB3; MIM 173470) complex and has E3 ubiquitin ligase activity (Brophy et al., 2008 [PubMed 18331836]).[supplied by OMIM, Dec 2008]
UniProt Code:Q9P0P0
NCBI GenInfo Identifier:74761852
NCBI Gene ID:51255
NCBI Accession:Q9P0P0.1
UniProt Related Accession:Q9P0P0
Molecular Weight:17kDa
NCBI Full Name:E3 ubiquitin-protein ligase RNF181
NCBI Synonym Full Names:ring finger protein 181
NCBI Official Symbol:RNF181��
NCBI Official Synonym Symbols:HSPC238��
NCBI Protein Information:E3 ubiquitin-protein ligase RNF181
UniProt Protein Name:E3 ubiquitin-protein ligase RNF181
UniProt Synonym Protein Names:RING finger protein 181
Protein Family:E3 ubiquitin-protein ligase
UniProt Gene Name:RNF181��
UniProt Entry Name:RN181_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9P0P0
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