Cell Cycle Recombinant Proteins
Human RhoA Recombinant Protein (RPES5083)
- SKU:
- RPES5083
Description
Human RhoA Recombinant Protein
Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.
| Product Name: | Human RhoA Recombinant Protein (RPES5083) |
| Product Code: | RPES5083 |
| Size: | 50µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | ARH12,ARHA,RHO12,RHOH12 |
| Accession: | P61586-1 |
| Sequence: | Met 1-Leu 193 |
| Fusion tag: | N-His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human RHOA (P61586-1) (Met 1-Leu 193) was fused with a polyhistidine tag at the N-terminus. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 24 kDa |
| AP Mol Mass: | 28 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Cell Cycle |
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