Human REN Recombinant Protein (RPPB4442)
- SKU:
- RPPB4442
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P00797
Description
| Product Name: | Human REN Recombinant Protein |
| Product Code: | RPPB4442 |
| Size: | 10µg |
| Species: | Human |
| Target: | REN |
| Synonyms: | Renin, Angiotensinogenase, EC 3.4.23.15, HNFJ2, Angiotensin-Forming Enzyme, Renin Precursor Renal, EC 3.4.23. |
| Source: | HEK293 Cells |
| Physical Appearance: | Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | REN was filtered (0.4�m) and lyophilized from 0.5mg/ml solution in phosphate buffered saline and 5% (w/v) trehalose. |
| Solubility: | It is recommended to add 200�l deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. REN is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. |
| Stability: | Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | LPTDTTTFKR IFLKRMPSIR ESLKERGVDM ARLGPEWSQP MKRLTLGNTT SSVILTNYMD TQYYGEIGIG TPPQTFKVVF DTGSSNVWVP SSKCSRLYTA CVYHKLFDAS DSSSYKHNGT ELTLRYSTGT VSGFLSQDII TVGGITVTQM FGEVTEMPAL PFMLAEFDGV VGMGFIEQAI GRVTPIFDNI ISQGVLKEDV FSFYYNRDSE NSQSLGGQIV LGGSDPQHYE GNFHYINLIK TGVWQIQMKG VSVGSSTLLC EDGCLALVDT GASYISGSTS SIEKLMEALG AKKRLFDYVV KCNEGPTLPD ISFHLGGKEY TLTSADYVFQ ESYSSKKLCT LAIHAMDIPP PTGPTWALGA TFIRKFYTEF DRRNNRIGFA LAR HHHHHHH HHH |
Renin is a highly specific endopeptidase which generates angiotensin I from angiotensinogen in the plasma. angiotensin I is an important regulator of blood pressure and electrolyte balance. Renin initiates a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
Renin Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (Leu24-Arg406) containing a total of 393 amino acids, having a calculated molecular mass of 43.7kDa and fused to a 10 aa His tag at C-Terminus.
| UniProt Protein Function: | REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; EC 3.4.23.15; Protease; Secreted Chromosomal Location of Human Ortholog: 1q32 Cellular Component: extracellular space; cytoplasm; plasma membrane; extracellular region Molecular Function:peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding Biological Process: response to cAMP; hormone-mediated signaling; renin-angiotensin regulation of aldosterone production; male gonad development; cell maturation; drinking behavior; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; beta-amyloid metabolic process; regulation of MAPKKK cascade; regulation of blood pressure; mesonephros development; kidney development Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2 |
| NCBI Summary: | Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P00797 |
| NCBI GenInfo Identifier: | 132326 |
| NCBI Gene ID: | 5972 |
| NCBI Accession: | P00797.1 |
| UniProt Secondary Accession: | P00797,Q6FI38, Q6T5C2, |
| UniProt Related Accession: | P00797 |
| Molecular Weight: | 44,726 Da |
| NCBI Full Name: | Renin |
| NCBI Synonym Full Names: | renin |
| NCBI Official Symbol: | REN�� |
| NCBI Official Synonym Symbols: | HNFJ2�� |
| NCBI Protein Information: | renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme |
| UniProt Protein Name: | Renin |
| UniProt Synonym Protein Names: | Angiotensinogenase |
| Protein Family: | Renin |
| UniProt Gene Name: | REN�� |
| UniProt Entry Name: | RENI_HUMAN |
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