Human RCHY1 Recombinant Protein (RPPB4428)
- SKU:
- RPPB4428
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q96PM5
Description
| Product Name: | Human RCHY1 Recombinant Protein |
| Product Code: | RPPB4428 |
| Size: | 20µg |
| Species: | Human |
| Target: | RCHY1 |
| Synonyms: | ARNIP, CHIMP, hARNIP, PIRH2, PIRH2E, PIRH2F, PRO1996, RNF199, ZNF363, RING finger and CHY zinc finger domain-containing protein 1, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2, RCHY1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RCHY1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAATARE DGASGQERGQ RGCEHYDRGC LLKAPCCDKL YTCRLCHDNN EDHQLDRFKV KEVQCINCEK IQHAQQTCEE CSTLFGEYYC DICHLFDKDK KQYHCENCGI CRIGPKEDFF HCLKCNLCLA MNLQGRHKCI ENVSRQNCPI CLEDIHTSRV VAHVLPCGHL LHRTCYEEML KEGYRCPLCM HSALDMTRYW RQLDDEVAQT PMPSEYQNMT VDILCNDCNG RSTVQFHILG MKCKICESYN TAQAGGRRIS LDQQ |
Ring Finger & CHY Zinc Finger Domain Containing 1 (RCHY1) acts as an ubiquitin ligase. RCHY1 mediates E3-dependent ubiquitination and proteasomal degradation of target proteins which among them are TP53, HDAC1 and CDKN1B, consequently regulating their levels and cell cycle progression. RCHY1 is also increases AR transcription factor activity.
RCHY1 Human Recombinant produced in E. coli is a single polypeptide chain containing 284 amino acids (1-261) and having a molecular mass of 32.5kDa. RCHY1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RCHY1: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and p27Kip1. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of p27Kip1 and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, p27Kip1, COPE, UBE2D2 and GORAB/NTKLBP1. Up-regulated during the S phase of the cell cycle. Expressed at low levels during G phase. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; Ubiquitin conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19 Chromosomal Location of Human Ortholog: 4q21.1 Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex Molecular Function:protein binding; protein homodimerization activity; zinc ion binding; p53 binding; ubiquitin-protein ligase activity; ligase activity; receptor binding Biological Process: protein autoubiquitination; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination |
| NCBI Summary: | The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q96PM5 |
| NCBI GenInfo Identifier: | 32700008 |
| NCBI Gene ID: | 25898 |
| NCBI Accession: | Q96PM5.1 |
| UniProt Related Accession: | Q96PM5 |
| Molecular Weight: | 30 kDa |
| NCBI Full Name: | RING finger and CHY zinc finger domain-containing protein 1 |
| NCBI Synonym Full Names: | ring finger and CHY zinc finger domain containing 1 |
| NCBI Official Symbol: | RCHY1�� |
| NCBI Official Synonym Symbols: | ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996�� |
| NCBI Protein Information: | RING finger and CHY zinc finger domain-containing protein 1 |
| UniProt Protein Name: | RING finger and CHY zinc finger domain-containing protein 1 |
| UniProt Synonym Protein Names: | Androgen receptor N-terminal-interacting protein; CH-rich-interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; Zinc finger protein 363; p53-induced RING-H2 protein; hPirh2 |
| Protein Family: | RING finger and CHY zinc finger domain-containing protein |
| UniProt Gene Name: | RCHY1�� |
| UniProt Entry Name: | ZN363_HUMAN |
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