Human RBM3 Recombinant Protein (RPPB4419)

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RPPB4419

Description

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Product Name:Human RBM3 Recombinant Protein
Product Code:RPPB4419
Size:20µg
Species:Human
Target:RBM3
Synonyms:IS1-RNPL, RNPL, RNA-binding motif protein 3, PutativeRNA-Binding Protein 3, RNA Binding Motif (RNP1, RRM) Protein 3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:RBM3protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2MNaCl, 50% glycerol and 5mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSSEEGK LFVGGLNFNT DEQALEDHFS SFGPISEVVV VKDRETQRSRGFGFITFTNP EHASVAMRAM NGESLDGRQI RVDHAGKSAR GTRGGGFGAH GRGRSYSRGG GDQGYGSGRYYDSRPGGYGY GYGRSRDYNG RNQGGYDRYS GGNYRDNYDN

RBM3belongs to the glycine-rich RNA-binding protein family and encodes a proteinwith one RNA recognition motif (RRM) domain. Bycold shock and low oxygen tension the expression of this gene is induced. Onchromosome 1 a pseudogene exists. Alternate transcriptional splice variants,encoding different isoforms, have beencharacterized. Diseases associated with RBM3include�vaccinia, andcryptorchidism. GO annotations linked to this geneinclude�RNA binding�and�ribosomal large subunit binding. Animportant paralog of this gene is�RBMY1J.

RBM3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 180 amino acids (1-157 a.a) and having a molecular mass of 19kDa.RBM3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RBM3: Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.
UniProt Protein Details:

Protein type:Translation; RNA-binding; Ribosomal; RNA processing

Chromosomal Location of Human Ortholog: Xp11.2

Cellular Component: cytoplasm; dendrite; nucleus; large ribosomal subunit

Molecular Function:protein binding; RNA binding; nucleotide binding; ribosomal large subunit binding

Biological Process: RNA processing; regulation of translation; miRNA-mediated gene silencing, production of miRNAs; positive regulation of translation; translation; response to cold

NCBI Summary:This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]
UniProt Code:P98179
NCBI GenInfo Identifier:1710620
NCBI Gene ID:5935
NCBI Accession:P98179.1
UniProt Related Accession:P98179
Molecular Weight:157
NCBI Full Name:Putative RNA-binding protein 3
NCBI Synonym Full Names:RNA binding motif (RNP1, RRM) protein 3
NCBI Official Symbol:RBM3��
NCBI Official Synonym Symbols:RNPL; IS1-RNPL��
NCBI Protein Information:putative RNA-binding protein 3; RNA-binding motif protein 3
UniProt Protein Name:Putative RNA-binding protein 3
UniProt Synonym Protein Names:RNA-binding motif protein 3; RNPL
Protein Family:RNA-binding protein
UniProt Gene Name:RBM3��
UniProt Entry Name:RBM3_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P98179
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