Human RBM17 Recombinant Protein (RPPB4422)

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SKU:
RPPB4422
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96I25

Description

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Product Name:Human RBM17 Recombinant Protein
Product Code:RPPB4422
Size:10µg
Species:Human
Target:RBM17
Synonyms:RNA Binding Motif Protein 17, SPF45, Splicing Factor 45kDa, RNA-Binding Motif Protein 17, Splicing Factor 45, 45 kDa-splicing factor, RBM17.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:RBM17 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MSLYDDLGVE TSDSKTEGWS KNFKLLQSQL QVKKAALTQA KSQRTKQSTV LAPVIDLKRG GSSDDRQIVD TPPHVAAGLK DPVPSGFSAG EVLIPLADEY DPMFPNDYEK VVKRQREERQ RQRELERQKE IEEREKRRKD RHEASGFARR PDPDSDEDED YERERRKRSM GGAAIAPPTS LVEKDKELPR DFPYEEDSRP RSQSSKAAIP PPVYEEQDRP RSPTGPSNSF LANMGGTVAH KIMQKYGFRE GQGLGKHEQG LSTALSVEKT SKRGGKIIVG DATEKDASKK SDSNPLTEIL KCPTKVVLLR NMVGAGEVDE DLEVETKEEC EKYGKVGKCV IFEIPGAPDD EAVRIFLEFE RVESAIKAVV DLNGRYFGGR VVKACFYNLD KFRVLDLAEQ V

RNA binding motif protein 17 (RBM17) is a splice factor which binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the 2nd catalytic step. The RBM17 protein is involved in the regulation of alternative splicing and the utilization of cryptic splice sites. RBM17 protein also stimulates the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The ensuing frameshift leads to sickle cell anemia.

RBM17 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 421 amino acids (1-401 a.a) and having a molecular mass of 47.1kDa.RBM17 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RBM17: Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
UniProt Protein Details:

Protein type:RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: nucleoplasm; spliceosome

Molecular Function:protein binding; RNA binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome

NCBI Summary:This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009]
UniProt Code:Q96I25
NCBI GenInfo Identifier:34925383
NCBI Gene ID:84991
NCBI Accession:Q96I25.1
UniProt Secondary Accession:Q96I25,Q96GY6,
UniProt Related Accession:Q96I25
Molecular Weight:401
NCBI Full Name:Splicing factor 45
NCBI Synonym Full Names:RNA binding motif protein 17
NCBI Official Symbol:RBM17��
NCBI Official Synonym Symbols:SPF45��
NCBI Protein Information:splicing factor 45; splicing factor 45kDa; 45 kDa-splicing factor; RNA-binding motif protein 17
UniProt Protein Name:Splicing factor 45
UniProt Synonym Protein Names:45 kDa-splicing factor; RNA-binding motif protein 17
Protein Family:Splicing factor
UniProt Gene Name:RBM17��
UniProt Entry Name:SPF45_HUMAN
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