Human RASSF1A Recombinant Protein (RPPB4413)

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SKU:
RPPB4413
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9NS23

Description

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Product Name:Human RASSF1A Recombinant Protein
Product Code:RPPB4413
Size:25µg
Species:Human
Target:RASSF1A
Synonyms:123F2, NORE2A, RASSF1, RDA32, REH3P21, Ras association domain-containing protein 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:RASSF1A protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.�
Amino Acid Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSGE PELIELRELA PAGRAGKGRT RLERANALRI ARGTACNPTR QLVPGRGHRF QPAGPATHTW CDLCGDFIWG VVRKGLQCAH CKFTCHYRCR ALVCLDCCGP RDLGWEPAVE RDTNVDEPVE WETPDLSQAE IEQKIKEYNA QINSNLFMSL NKDGSYTGFI KVQLKLVRPV SVPSSKKPPS LQDARRGPGR GTSVRRRTSF YLPKDAVKHL HVLSRTRARE VIEALLRKFL VVDDPRKFAL FERAERHGQV YLRKLLDDEQ PLRLRLLAGP SDKALSFVLK ENDSGEVNWD AFSMPELHNF LRILQREEEE HLRQILQKYS YCRQKIQEAL HACPLG

RASSF1A protein is a potential tumor suppressor like the RAS effector proteins. Presence or absence expression of this gene has been related with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this RASSF1A gene. The inactivation of RASSF1A was found to be associated with the hypermethylation of its CpG-island promoter region. RASSF1A required for death receptor-dependent apoptosis.

RASSF1A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 376 amino acids (1-340 a.a.) and having a molecular mass of 42.9kDa. RASSF1A is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RASSF1: Potential tumor suppressor. Required for death receptor- dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. Interacts with MAP1S and XPA. Binds to the N-terminal of CDC20 during prometaphase. Binds to STK3/MST2 and STK4/MST1. Isoform A interacts with MOAP1 and E4F1. Recruited to the TNFRSF1A and TNFRSF10A complexes in response to their respective cognate ligand, after internalization. Can self-associate. Isoform A interacts with RSSF5 and probably associates with HRAS1 via a RSSF1 isoform A-RSSF5 heterodimer. Part of a complex with MDM2, DAXX, RASSF1 and USP7. Isoform A interacts (via C-terminus) with DAXX (via N-terminus); the interaction is independent of MDM2 and TP53. Isoform C or isoform H interacts (via N-terminus) with DAXX. Isoform A interacts (via N-terminus) with MDM2 (via C-terminus); the interaction is independent of TP53. Isoform A and isoform C are ubiquitously expressed in all tissues tested, however isoform A is absent in many corresponding cancer cell lines. Isoform B is mainly expressed in hematopoietic cells. 8 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:Tumor suppressor

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; microtubule cytoskeleton; nucleus; plasma membrane

Molecular Function:protein binding; zinc ion binding

Biological Process: cell cycle arrest; Ras protein signal transduction

Disease: Lung Cancer

NCBI Summary:This gene encodes a protein similar to the RAS effector proteins. Loss or altered expression of this gene has been associated with the pathogenesis of a variety of cancers, which suggests the tumor suppressor function of this gene. The inactivation of this gene was found to be correlated with the hypermethylation of its CpG-island promoter region. The encoded protein was found to interact with DNA repair protein XPA. The protein was also shown to inhibit the accumulation of cyclin D1, and thus induce cell cycle arrest. Several alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Code:Q9NS23
NCBI GenInfo Identifier:50401686
NCBI Gene ID:11186
NCBI Accession:Q9NS23.1
UniProt Secondary Accession:Q9NS23,O14571, O60539, O60710, Q0VGC6, Q5TZT2, Q9HB04 Q9HB18, Q9NS22, Q9UND4, Q9UND5, B7ZLL1,
UniProt Related Accession:Q9NS23
Molecular Weight:8,656 Da
NCBI Full Name:Ras association domain-containing protein 1
NCBI Synonym Full Names:Ras association domain family member 1
NCBI Official Symbol:RASSF1��
NCBI Official Synonym Symbols:123F2; RDA32; NORE2A; RASSF1A; REH3P21��
NCBI Protein Information:ras association domain-containing protein 1
UniProt Protein Name:Ras association domain-containing protein 1
Protein Family:Ras association domain-containing protein
UniProt Gene Name:RASSF1��
UniProt Entry Name:RASF1_HUMAN
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