Human RAP2B Recombinant Protein (RPPB4403)
- SKU:
- RPPB4403
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P61225
Description
| Product Name: | Human RAP2B Recombinant Protein |
| Product Code: | RPPB4403 |
| Size: | 20µg |
| Species: | Human |
| Target: | RAP2B |
| Synonyms: | RAP2B, member of RAS oncogene family, Ras-related protein Rap-2b, Ras Family Small GTP Binding Protein RAP2B2, Small GTP Binding Protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | RAP2B protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMREYKVV VLGSGGVGKS ALTVQFVTGS FIEKYDPTIE DFYRKEIEVD SSPSVLEILD TAGTEQFASM RDLYIKNGQG FILVYSLVNQ QSFQDIKPMR DQIIRVKRYE RVPMILVGNK VDLEGEREVS YGEGKALAEE WSCPFMETSA KNKASVDELF AEIVRQMNYA AQPNGDEGCC SACVIL |
Ras-related protein Rap-2b (RAP2B) is a part of RAS-related genes family. RAP2B has a threonine residue at aa 61Unlike normal ras proteins which have a nontransforming glutamine residue at that position. The threonine residue makes the intrinsic GTPase activity for Rap2B lower, by this means allows RAP2B to exist in the activated state for a longer period of time than normal ras proteins. RAP2B is a platelet protein which is activated by thrombin and involved in platelet activation. RAP2B may be a candidate oncogene which takes part in carcinogenesis through activation of NF-kappaB pathway.
RAP2B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-183 a.a) and having a molecular mass of 22.9kDa. RAP2B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAP2B: Small GTP-binding protein which cycles between a GDP- bound inactive and a GTP-bound active form. Involved in EGFR and CHRM3 signaling pathways through stimulation of PLCE1. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May regulate membrane vesiculation in red blood cells. Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 3q25.2 Cellular Component: tight junction; membrane; recycling endosome membrane; plasma membrane; cytosol; lipid raft Molecular Function:protein domain specific binding; GDP binding; GTP binding Biological Process: platelet activation; microvillus biogenesis; signal transduction; positive regulation of protein amino acid autophosphorylation; negative regulation of cell migration; Rap protein signal transduction |
| NCBI Summary: | This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61225 |
| NCBI GenInfo Identifier: | 47117762 |
| NCBI Gene ID: | 5912 |
| NCBI Accession: | P61225.1 |
| UniProt Secondary Accession: | P61225,P17964, Q96EG5, Q9CXG0, |
| UniProt Related Accession: | P61225 |
| Molecular Weight: | 183 |
| NCBI Full Name: | Ras-related protein Rap-2b |
| NCBI Synonym Full Names: | RAP2B, member of RAS oncogene family |
| NCBI Official Symbol: | RAP2B�� |
| NCBI Protein Information: | ras-related protein Rap-2b; small GTP binding protein |
| UniProt Protein Name: | Ras-related protein Rap-2b |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAP2B�� |
| UniProt Entry Name: | RAP2B_HUMAN |
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