Human RAP1B Recombinant Protein (RPPB4402)
- SKU:
- RPPB4402
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P61224
Description
| Product Name: | Human RAP1B Recombinant Protein |
| Product Code: | RPPB4402 |
| Size: | 10µg |
| Species: | Human |
| Target: | RAP1B |
| Synonyms: | RAP1B, member of RAS oncogene family, DKFZp586H0723, K-REV, RAL1B, GTP-binding protein smg p21B, Ras family small GTP binding protein RAP1B, RAS-related protein RAP1B, small GTP binding protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RAP1B protein solution (0.25mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINRKTP VPGKARKKSS C |
RAP1B is a member of a superfamily of RAS-like small GTP-binding proteins which takes part in cell signaling. RAP1B owns an intrinsic GTPase activity and is universally expressed in mammalian tissues. The alleged effector domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins.
RAP1B produced in E.Coli is a single, non-glycosylated polypeptide chain containing�201 amino acids (1-181.a.a) and having a molecular mass of 22.6kDa. RAP1B is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAP1B: GTP-binding protein that possesses intrinsic GTPase activity. Contributes to the polarizing activity of KRIT1 and CDH5 in the establishment and maintenance of correct endothelial cell polarity and vascular lumen. Required for the localization of phosphorylated PRKCZ, PARD3 and TIAM1 to the cell junction. Heterodimer with RAP1GAP. Interacts with EPAC2, SGSM1, SGSM2 and SGSM3. Activated by binding to the GTPase-activating protein RAP1GAP. Activated by guanine nucleotide-exchange factor (GEF) EPAC2 in a cAMP-dependent manner. Belongs to the small GTPase superfamily. Ras family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, Ras; G protein, monomeric; G protein Chromosomal Location of Human Ortholog: 12q14 Cellular Component: membrane; plasma membrane; lipid particle; intracellular; intercellular junction; cytosol; lipid raft Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; protein complex binding Biological Process: platelet activation; cell proliferation; microvillus biogenesis; negative regulation of calcium ion-dependent exocytosis; metabolic process; blood coagulation; Rap protein signal transduction |
| NCBI Summary: | This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P61224 |
| NCBI GenInfo Identifier: | 47117723 |
| NCBI Gene ID: | 5908 |
| NCBI Accession: | P61224.1 |
| UniProt Related Accession: | P61224 |
| Molecular Weight: | |
| NCBI Full Name: | Ras-related protein Rap-1b |
| NCBI Synonym Full Names: | RAP1B, member of RAS oncogene family |
| NCBI Official Symbol: | RAP1B�� |
| NCBI Official Synonym Symbols: | K-REV; RAL1B�� |
| NCBI Protein Information: | ras-related protein Rap-1b |
| UniProt Protein Name: | Ras-related protein Rap-1b |
| UniProt Synonym Protein Names: | GTP-binding protein smg p21B |
| Protein Family: | Ras-related protein |
| UniProt Gene Name: | RAP1B�� |
| UniProt Entry Name: | RAP1B_HUMAN |
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