Growth Factors & Cytokines Recombinant Proteins
Human RANKL/TNFSF11 Recombinant Protein (RPES3610)
- SKU:
- RPES3610
Description
Human RANKL/TNFSF11 Recombinant Protein
CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-?B (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NF?B, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.
| Product Name: | Human RANKL/TNFSF11 Recombinant Protein (RPES3610) |
| Product Code: | RPES3610 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | CD254, ODF, OPGL, RANKL, TNFSF11, CD254, Osteoclast differentiation factor, Receptor activator of nuclear factor kappa-B ligand, tumor necrosis factor ligand superfamily member 11,hRANKL2,OPTB2,RANKL,sOdf |
| Accession: | O14788 |
| Sequence: | Ile140-Asp317 |
| Fusion tag: | N-6His |
| Activity: | Immobilized ZRKLA at 2µg/ml(100 ?l/well) can bind Human OPG-His(Cat: PKSH033573) The ED50 of ZRKLA is 0.15 ug/ml . |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Receptor Activator of NF-kappa-B ligand is produced by our E.coli expression system and the target gene encoding Ile140-Asp317 is expressed with a 6His tag at the N-terminus. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 22.4 kDa |
| AP Mol Mass: | 20 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM Tris,150mM NaCl,pH8.0. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Growth Factors & Cytokines |
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