Human RANBP3 Recombinant Protein (RPPB4399)

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SKU:
RPPB4399
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H6Z4

Description

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Product Name:Human RANBP3 Recombinant Protein
Product Code:RPPB4399
Size:20µg
Species:Human
Target:RANBP3
Synonyms:RAN binding protein 3, RanBP3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:RANBP3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSISSSLEN STNSADASSN KFVFGQNMSE RVLSPPKLNE VSSDANRENA AAESGSESSS QEATPEKESL AESAAAYTKA TARKCLLEKV EVITGEEAES NVLQMQCKLF VFDKTSQSWV ERGRGLLRLN DMASTDDGTL QSRLVMRTQG SLRLILNTKL WAQMQIDKAS EKSIRITAMD TEDQGVKVFL ISASSKDTGQ LYAALHHRIL ALRS

RAN Binding Protein 3, also known as RANBP3, is found in the nucleus and cytoplasm. RANBP3 which owns RanBD1 domain participates in nuclear export as part of a heteromeric complex. RANBP3 is also negatively regulates TGF-beta signaling by interacting with the R-SMAD proteins, SMAD2 and SMAD3.

RANBP3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (235-445 a.a) and having a molecular mass of 25.5kDa. RANBP3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RANBP3: Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF- beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export. Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling. Widely expressed with high levels in testis and heart. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: positive regulation of protein export from nucleus

NCBI Summary:This gene encodes a protein with a RanBD1 domain that is found in both the nucleus and cytoplasm. This protein plays a role in nuclear export as part of a heteromeric complex. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:Q9H6Z4
NCBI GenInfo Identifier:51316528
NCBI Gene ID:8498
NCBI Accession:Q9H6Z4.1
UniProt Secondary Accession:Q9H6Z4,O60405, O75759, O75760, Q9BT47, Q9UG74, B2RAT8
UniProt Related Accession:Q9H6Z4
Molecular Weight:53,411 Da
NCBI Full Name:Ran-binding protein 3
NCBI Synonym Full Names:RAN binding protein 3
NCBI Official Symbol:RANBP3��
NCBI Protein Information:ran-binding protein 3
UniProt Protein Name:Ran-binding protein 3
Protein Family:Ran-binding protein
UniProt Gene Name:RANBP3��
UniProt Entry Name:RANB3_HUMAN
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