Human RAD51B Recombinant Protein (RPPB4383)
- SKU:
- RPPB4383
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O15315
Description
| Product Name: | Human RAD51B Recombinant Protein |
| Product Code: | RPPB4383 |
| Size: | 20µg |
| Species: | Human |
| Target: | RAD51B |
| Synonyms: | RAD51 homolog B (S. cerevisiae), R51H2, RAD51L1, REC2, DNA repair protein RAD51 homolog 2, Rad51B, RAD51-like protein 1, RAD51B. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RAD51B solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 0.4M Urea. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMGSKKLK RVGLSQELCD RLSRHQILTC QDFLCLSPLE LMKVTGLSYR GVHELLCMVS RACAPKMQTA YGIKAQRSAD FSPAFLSTTL SALDEALHGG VACGSLTEIT GPPGCGKTQF CIMMSILATL PTNMGGLEGA VVYIDTESAF SAERLVEIAE SRFPRYFNTE EKLLLTSSKV HLYRELTCDE VLQRIESLEE EIISKGIKLV ILDSVASVVR KEFDAQLQGN LKERNKFLAR EASSLKYLAE EFSIPVILTN QITTHLSGAL ASQADLVSPA DDLSLSEGTS GSSCVIAALG NTWSHSVNTR LILQYLDSER RQILIAKSPL APFTSFVYTI KEEGLVLQAY GNS |
RAD51 family members are evolutionarily conserved proteins vital for DNA repair by homologous recombination. RAD51B is a part of the RAD51 protein family. RAD51B is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks occurring during DNA replication or induced by DNA-damaging agents. RAD51B forms a stable heterodimer with the family member RAD51C, which interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of RAD51B leads to cell cycle G1 delay and cell apoptosis.
RAD51B Human Recombinant produced in E. coli is. a single polypeptide chain containing 373 amino acids (1-350) and having a molecular mass of 40.6kDa. RAD51B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RAD51L1: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. The RAD51B- RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single- stranded gaps in duplex DNA and specifically to nicks in duplex DNA. A chromosomal aberration involving RAD51B is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23- 24) with HMGA1. A chromosomal aberration involving RAD51B is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2. Belongs to the RecA family. RAD51 subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 14q23-q24.2 Cellular Component: nucleoplasm; replication fork; nucleus Molecular Function:DNA-dependent ATPase activity; protein binding; four-way junction DNA binding; DNA binding; double-stranded DNA binding; single-stranded DNA binding; ATP binding Biological Process: meiotic recombination; blood coagulation; DNA repair; double-strand break repair via homologous recombination; DNA recombination Disease: Leiomyoma, Uterine |
| NCBI Summary: | The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata. [provided by RefSeq, Jul 2011] |
| UniProt Code: | O15315 |
| NCBI GenInfo Identifier: | 38258892 |
| NCBI Gene ID: | 5890 |
| NCBI Accession: | O15315.2 |
| UniProt Secondary Accession: | O15315,O60914, O75210, Q3Y4F8, Q6FHX8, Q86SY3, Q86SY4 Q86TR0, Q86U92, Q86U93, Q86U94, Q8N6H4, |
| UniProt Related Accession: | O15315 |
| Molecular Weight: | 384 |
| NCBI Full Name: | DNA repair protein RAD51 homolog 2 |
| NCBI Synonym Full Names: | RAD51 paralog B |
| NCBI Official Symbol: | RAD51B�� |
| NCBI Official Synonym Symbols: | REC2; R51H2; RAD51L1�� |
| NCBI Protein Information: | DNA repair protein RAD51 homolog 2; RAD51 homolog B; RecA-like protein; recombination repair protein |
| UniProt Protein Name: | DNA repair protein RAD51 homolog 2 |
| UniProt Synonym Protein Names: | RAD51 homolog B; Rad51B; RAD51-like protein 1 |
| UniProt Gene Name: | RAD51B�� |
| UniProt Entry Name: | RA51B_HUMAN |
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