Human RAB35 Recombinant Protein (RPPB4362)

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SKU:
RPPB4362
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15286

Description

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Product Name:Human RAB35 Recombinant Protein
Product Code:RPPB4362
Size:10µg
Species:Human
Target:RAB35
Synonyms:Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:RAB35 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MARDYDHLFK LLIIGDSGVG KSSLLLRFAD NTFSGSYITT IGVDFKIRTV EINGEKVKLQ IWDTAGQERF RTITSTYYRG THGVIVVYDV TSAESFVNVK RWLHEINQNC DDVCRILVGN KNDDPERKVV ETEDAYKFAG QMGIQLFETS AKENVNVEEM FNCITELVLR AKKDNLAKQQ QQQQNDVVKL TKNSKRKKRC C

Ras-related protein Rab-35 (RAB35) is restricted to the plasma membrane and endocytic compartments and controls a fast endocytic recycling pathway. Inhibition of Rab35 function leads to the accumulation of endocytic markers on numerous cytoplasmic vacuoles in cells that failed cytokinesis, which is consistent with a central requirement for Rab35-regulated recycling during cell division.

RAB35 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 221 amino acids (1-201 a.a.) and having a molecular mass of 25.2kDa.RAB35 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RAB35: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Interacts with DENND1A and DENND1B in a nucleotide- dependent manner. The low binding to DENND1C is nucleotide- independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell projection membrane; clathrin-coated endocytic vesicle; coated pit; endoplasmic reticulum membrane; endosome membrane; Golgi membrane; intercellular bridge; plasma membrane

Molecular Function:GDP binding; GTP binding; GTPase activity; phosphatidylinositol-4,5-bisphosphate binding; protein binding

Biological Process: antigen processing and presentation; cytokinesis; endocytic recycling; endosome transport; ER to Golgi vesicle-mediated transport; neurite development; plasma membrane to endosome transport; protein localization

UniProt Code:Q15286
NCBI GenInfo Identifier:3024525
NCBI Gene ID:11021
NCBI Accession:Q15286.1
UniProt Secondary Accession:Q15286,B2R6E0, B4E390,
UniProt Related Accession:Q15286
Molecular Weight:17,058 Da
NCBI Full Name:Ras-related protein Rab-35
NCBI Synonym Full Names:RAB35, member RAS oncogene family
NCBI Official Symbol:RAB35��
NCBI Official Synonym Symbols:RAY; H-ray; RAB1C��
NCBI Protein Information:ras-related protein Rab-35
UniProt Protein Name:Ras-related protein Rab-35
UniProt Synonym Protein Names:GTP-binding protein RAY; Ras-related protein Rab-1C
Protein Family:Ras-related protein
UniProt Gene Name:RAB35��
UniProt Entry Name:RAB35_HUMAN
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