Enzymes Recombinant Proteins

Human PYCRL Recombinant Protein (RPPB2234)

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RPPB2234

Description

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Product Name:Human PYCRL Recombinant Protein
Product Code:RPPB2234
Size:10µg
Species:Human
Target:PYCRL
Synonyms:Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein, PYCRL.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PYCRL solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAAEPS PRRVGFVGAG RMAGAIAQGL IRAGKVEAQH ILASAPTDRN LCHFQALGCR TTHSNQEVLQ SCLLVIFATK PHVLPAVLAE VAPVVTTEHI LVSVAAGVSL STLEELLPPN TRVLRVLPNL PCVVQEGAIV MARGRHVGSS ETNLLQHLLE ACGRCEEVPE AYVDIHTGLS GSGVAFVCAF SEALAEGAVK MGMPSSLAHR IAAQTLLGTA KMLLHEGQHP AQLRSDVCTP GGTTIYGLHA LEQGGLRAAT MSAVEAATCR AKELSRK

Pyrroline-5-Carboxylate Reductase Like (PYCRL) is a member of the pyrroline-5-carboxylate reductase family and acts as a homodecamer. PYCRL plays a key role in proline bio-synthesis. Proline serves as a non-enzymatic antioxidant to reduce damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the final stage of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline with NAD(P)H as the cofactor.

PYCRL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (1-274) and having a molecular mass of 31kDa.PYCRL is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PYCRL: Belongs to the pyrroline-5-carboxylate reductase family.
UniProt Protein Details:

Protein type:Hydrolase; Oxidoreductase; EC 1.5.1.2; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function:protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: amino acid biosynthetic process

UniProt Code:Q53H96
NCBI GenInfo Identifier:727863586
NCBI Gene ID:65263
NCBI Accession:Q53H96.3
UniProt Secondary Accession:Q53H96,Q8N3N9, Q96HX4, Q9H896, B3KMB5, B4DVT6, H0Y6C3
UniProt Related Accession:Q53H96
Molecular Weight:26,440 Da
NCBI Full Name:Pyrroline-5-carboxylate reductase 3
NCBI Synonym Full Names:pyrroline-5-carboxylate reductase-like
NCBI Official Symbol:PYCRL��
NCBI Official Synonym Symbols:PYCR3��
NCBI Protein Information:pyrroline-5-carboxylate reductase 3
UniProt Protein Name:Pyrroline-5-carboxylate reductase 3
UniProt Synonym Protein Names:Pyrroline-5-carboxylate reductase-like protein
UniProt Gene Name:PYCRL��
UniProt Entry Name:P5CR3_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q53H96
Research Area:
Enzymes
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