Enzymes Recombinant Proteins

Human PYCR2 Recombinant Protein (RPPB2233)

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RPPB2233

Description

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Product Name:Human PYCR2 Recombinant Protein
Product Code:RPPB2233
Size:20µg
Species:Human
Target:PYCR2
Synonyms:P5CR2, Pyrroline-5-carboxylate reductase 2 isoform 1,P5C reductase 2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PYCR2 protein solution (0.25mg/ml) in phosphate buffered saline (pH7.4), 50% glycerol, 5mM DTT and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRKMGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKVIRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDALADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINAVEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD

Pyrroline-5-carboxylate reductase 2 isoform 1 (PYCR2) is a member of the pyrroline-5-carboxylate reductase family. PYCR2 protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The three substrates of the PYCR2 enzyme are L-proline, NAD+, and NADP+, while its four products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+.

PYCR2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-320 a.a) and having a molecular mass of 36kDa.PYCR2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PYCR2: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+). Can utilize both NAD and NADP. Has higher affinity for NADP, but higher catalytic efficiency with NADH. Belongs to the pyrroline-5-carboxylate reductase family.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.5.1.2

Chromosomal Location of Human Ortholog: 1q42.12

Cellular Component: cytoplasm

Molecular Function:pyrroline-5-carboxylate reductase activity

Disease: Leukodystrophy, Hypomyelinating, 10

NCBI Summary:This gene belongs to the pyrroline-5-carboxylate reductase family. The encoded mitochondrial protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]
UniProt Code:Q96C36
NCBI GenInfo Identifier:60390642
NCBI Gene ID:29920
NCBI Accession:Q96C36.1
UniProt Related Accession:Q96C36
Molecular Weight:
NCBI Full Name:Pyrroline-5-carboxylate reductase 2
NCBI Synonym Full Names:pyrroline-5-carboxylate reductase 2
NCBI Official Symbol:PYCR2��
NCBI Official Synonym Symbols:HLD10; P5CR2��
NCBI Protein Information:pyrroline-5-carboxylate reductase 2
UniProt Protein Name:Pyrroline-5-carboxylate reductase 2
Protein Family:Pyrroline-5-carboxylate reductase
UniProt Gene Name:PYCR2��
UniProt Entry Name:P5CR2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q96C36
Research Area:
Enzymes
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