Enzymes Recombinant Proteins

Human PTGES2 Recombinant Protein (RPPB2220)

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RPPB2220

Description

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Product Name:Human PTGES2 Recombinant Protein
Product Code:RPPB2220
Size:20µg
Species:Human
Target:PTGES2
Synonyms:Prostaglandin E synthase 2, Microsomal prostaglandin E synthase 2, mPGES-2, PTGES2, C9orf15, PGES2, GBF1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PTGES2 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 2mM DTT and 0.2M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MKAVNEQGKE VTEFGNKYWL MLNEKEAQQV YGGKEARTEE MKWRQWADDW LVHLISPNVY RTPTEALASF DYIVREGKFG AVEGAVAKYM GAAAMYLISK RLKSRHRLQD NVREDLYEAA DKWVAAVGKD RPFMGGQKPN LADLAVYGVL RVMEGLDAFD DLMQHTHIQP WYLRVERAIT EASPAH

PTGES2 is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. Additionally, PTGES2 activates the transcription regulated by a gamma-INF-activated transcription element. PTGES2 is widely expressed- in the heart, including apex, inter-ventricular septum, both atria and ventricles, but not in the aorta. PTGES2 is also expressed in fetal heart. PTGES2 is detected in various regions of the brain: cerebellum; occipital, frontal and parietal lobes. It is also expressed in the lymph nodes, skeletal muscle, kidney and trachea, but not in the thymus or lung. PTGES2 is overexpressed in colorectal cancer.

PTGES2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-186 a.a.) and having a molecular mass of 23.5kDa.PTGES2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PTGES2: Isomerase that catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. May also have transactivation activity toward IFN-gamma (IFNG), possibly via an interaction with CEBPB; however, the relevance of transcription activation activity remains unclear. Belongs to the GST superfamily.
UniProt Protein Details:

Protein type:Isomerase; EC 5.3.99.3; Lipid Metabolism - arachidonic acid; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q34.11

Cellular Component: Golgi membrane; mitochondrion; perinuclear region of cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function:DNA binding; glutathione binding; electron carrier activity; lyase activity; heme binding; protein disulfide oxidoreductase activity; prostaglandin-E synthase activity

Biological Process: secretion; cell redox homeostasis; positive regulation of transcription, DNA-dependent; prostaglandin biosynthetic process

NCBI Summary:The protein encoded by this gene is a membrane-associated prostaglandin E synthase, which catalyzes the conversion of prostaglandin H2 to prostaglandin E2. This protein also has been shown to activate the transcription regulated by a gamma-interferon-activated transcription element (GATE). Multiple transcript variants have been found for this gene. [provided by RefSeq, Jun 2009]
UniProt Code:Q9H7Z7
NCBI GenInfo Identifier:73921741
NCBI Gene ID:80142
NCBI Accession:Q9H7Z7.1
UniProt Secondary Accession:Q9H7Z7,Q53EW9, Q5SYV6, Q96GI0, Q96GL2,
UniProt Related Accession:Q9H7Z7
Molecular Weight:
NCBI Full Name:Prostaglandin E synthase 2
NCBI Synonym Full Names:prostaglandin E synthase 2
NCBI Official Symbol:PTGES2��
NCBI Official Synonym Symbols:GBF1; GBF-1; PGES2; C9orf15; mPGES-2��
NCBI Protein Information:prostaglandin E synthase 2; GATE-binding factor 1; gamma-interferon-activated transcriptional element-binding factor 1; mPGE synthase-2; membrane-associated prostaglandin E synthase 2; membrane-associated prostaglandin E synthase-2; microsomal prostaglandin E synthase-2; prostaglandin-H(2) E-isomerase
UniProt Protein Name:Prostaglandin E synthase 2
UniProt Synonym Protein Names:Membrane-associated prostaglandin E synthase-2
UniProt Gene Name:PTGES2��
UniProt Entry Name:PGES2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9H7Z7
Research Area:
Enzymes
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