Enzymes Recombinant Proteins

Human PSMD9 Recombinant Protein (RPPB2215)

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SKU:
RPPB2215
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00233
Research Area:
Enzymes

Description

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Product Name:Human PSMD9 Recombinant Protein
Product Code:RPPB2215
Size:20µg
Species:Human
Target:PSMD9
Synonyms:p27, Rpn4, 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, PSMD9.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMD9 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR

PSMD9 is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. PSMD9 acts as a chaperone through the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex compilation, PSMD9 is part of an intermediate PSMD9:PSMC6:PSMC3 module, also called modulator trimer complex. PSMD9 is released during the further base assembly process.

PSMD9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-223) and having a molecular mass of 27.1kDa. PSMD9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMD9: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Belongs to the proteasome subunit p27 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 12q24.31-q24.32

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function:bHLH transcription factor binding; protein binding; transcription coactivator activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of insulin secretion; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of insulin secretion; positive regulation of transcription, DNA-dependent; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway

NCBI Summary:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:O00233
NCBI GenInfo Identifier:317373521
NCBI Gene ID:5715
NCBI Accession:O00233.3
UniProt Secondary Accession:O00233,Q9BQ42, B2RD35, G3V1Q6,
UniProt Related Accession:O00233
Molecular Weight:13,053 Da
NCBI Full Name:26S proteasome non-ATPase regulatory subunit 9
NCBI Synonym Full Names:proteasome 26S subunit, non-ATPase 9
NCBI Official Symbol:PSMD9��
NCBI Official Synonym Symbols:p27; Rpn4��
NCBI Protein Information:26S proteasome non-ATPase regulatory subunit 9
UniProt Protein Name:26S proteasome non-ATPase regulatory subunit 9
UniProt Synonym Protein Names:26S proteasome regulatory subunit p27
Protein Family:26S proteasome non-ATPase regulatory
UniProt Gene Name:PSMD9��
UniProt Entry Name:PSMD9_HUMAN
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