Enzymes Recombinant Proteins

Human PSMD5 Recombinant Protein (RPPB2213)

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SKU:
RPPB2213
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16401
Research Area:
Enzymes

Description

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Product Name:Human PSMD5 Recombinant Protein
Product Code:RPPB2213
Size:20µg
Species:Human
Target:PSMD5
Synonyms:PSMD5, Proteasome (Prosome, Macropain) 26S Subunit,Non-ATPase, 526S Protease Subunit S5 Basic,26S Proteasome SubunitS5B,S5B,26S Proteasome Non-ATPase Regulatory Subunit 5,KIAA0072.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PSMD5 protein solution (1mg/ml) containing PhosphateBuffered Saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSEFMAAQA LALLREVARL EAPLEELRAL HSVLQAVPLN ELRQQAAELRLGPLFSLLNE NHREKTTLCV SILERLLQAM EPVHVARNLR VDLQRGLIHP DDSVKILTLS QIGRIVENSDAVTEILNNAE LLKQIVYCIG GENLSVAKAA IKSLSRISLT QAGLEALFES NLLDDLKSVM KTNDIVRYRVYELIIEISSV SPESLNYCTT SGLVTQLLRE LTGEDVLVRA TCIEMVTSLA YTHHGRQYLA QEGVIDQISNIIVGADSDPF SSFYLPGFVK FFGNLAVMDS PQQICERYPI FVEKVFEMIE SQDPTMIGVA VDTVGILGSNVEGKQVLQKT GTRFERLLMR IGHQSKNAPVELKIRCLDAI SSLLYLPPEQ QTDDLLRMTE SWFSSLSRDP LELFRGISSQ PFPELHCAAL KVFTAIANQP WAQKLMFNSPGFVEYVVDRS VEHDKASKDA KYELVKALAN SKTIAEIFGN PNYLRLRTYL SEGPYYVKPV STTAVEGAE

Proteasome26S Subunit, Non-ATPase 5, also known as PSMD5 is a member of the proteasomesubunit S5B/HSM3 family. The 26S proteasome is an enzymatic complex whichdegrades ubiquitinated proteins in eukaryotic cells. Furthermore, PSMD5 acts asa chaperones which is involved in the assembly of the 26s proteasome, particularlyof the base subcomplex of the PA700/19S regulatory complex. PSMD5 is full ofdileucine repeats, which have been involved in trafficking of various transmembraneproteins.

PSMD5 Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 529 amino acids (1-504 a.a) andhaving a molecular mass of 58.9kDa.PSMD5 is fused to a 25 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMD5: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD5:PSMC2:PSMC1:PSMD2 module which probably assembles with a PSMD10:PSMC4:PSMC5:PAAF1 module followed by dissociation of PSMD5. Belongs to the proteasome subunit S5B/HSM3 family.
UniProt Protein Details:

Protein type:Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: nucleoplasm; proteasome complex; cytosol

Molecular Function:protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

NCBI Summary:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a non-ATPase subunit of the 19S regulator base that functions as a chaperone protein during 26S proteasome assembly. [provided by RefSeq, Jul 2012]
UniProt Code:Q16401
NCBI GenInfo Identifier:3122657
NCBI Gene ID:5711
NCBI Accession:Q16401.3
UniProt Related Accession:Q16401
Molecular Weight:
NCBI Full Name:26S proteasome non-ATPase regulatory subunit 5
NCBI Synonym Full Names:proteasome 26S subunit, non-ATPase 5
NCBI Official Symbol:PSMD5��
NCBI Official Synonym Symbols:S5B��
NCBI Protein Information:26S proteasome non-ATPase regulatory subunit 5
UniProt Protein Name:26S proteasome non-ATPase regulatory subunit 5
UniProt Synonym Protein Names:26S protease subunit S5 basic; 26S proteasome subunit S5B
Protein Family:26S proteasome non-ATPase regulatory
UniProt Gene Name:PSMD5��
UniProt Entry Name:PSMD5_HUMAN
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