Enzymes Recombinant Proteins

Human PSMD13 Recombinant Protein (RPPB2217)

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SKU:
RPPB2217
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UNM6
Research Area:
Enzymes

Description

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Product Name:Human PSMD13 Recombinant Protein
Product Code:RPPB2217
Size:20µg
Species:Human
Target:PSMD13
Synonyms:HSPC027, p40.5, Rpn9, S11, 26S proteasome non-ATPase regulatory subunit 13, 26S proteasome regulatory subunit RPN9, 26S proteasome regulatory subunit S11, 26S proteasome regulatory subunit p40.5, PSMD13.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMD13 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M UREA and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMKDVPGF LQQSQNSGPG QPAVWHRLEE LYTKKLWHQL TLQVLDFVQD PCFAQGDGLI KLYENFISEF EHRVNPLSLV EIILHVVRQM TDPNVALTFL EKTREKVKSS DEAVILCKTA IGALKLNIGD LQVTKETIED VEEMLNNLPG VTSVHSRFYD LSSKYYQTIG NHASYYKDAL RFLGCVDIKD LPVSEQQERA FTLGLAGLLG EGVFNFGELL MHPVLESLRN TDRQWLIDTL YAFNSGNVER FQTLKTAWGQ QPDLAANEAQ LLRKIQLLCL MEMTFTRPAN HRQLTFEEIA KSAKITVNEV ELLVMKALSV GLVKGSIDEV DKRVHMTWVQ PRVLDLQQIK GMKDRLEFWC TDVKSMEMLV EHQAHDILT

Proteasome 26S Subunit, Non-ATPase 13 (PSMD13) is a part of the proteasome subunit S11 family and contains one PCI domain. PSMD13 plays a role as a regulatory subunit of the 26S proteasome which takes part in the ATP-dependent degradation of ubiquitinated proteins.

PSMD13 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 399 amino acids (1-376a.a) and having a molecular mass of 45.3kDa. PSMD13 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMD13: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S11 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytosol; extracellular region; membrane; nucleoplasm; nucleus; proteasome complex; proteasome regulatory particle

Molecular Function:structural molecule activity

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; neutrophil degranulation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; proteasome assembly; protein deubiquitination; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; ubiquitin-dependent protein catabolic process; Wnt receptor signaling pathway, planar cell polarity pathway

NCBI Summary:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UNM6
NCBI GenInfo Identifier:317373273
NCBI Gene ID:5719
NCBI Accession:Q9UNM6.2
UniProt Secondary Accession:Q9UNM6,O75831, Q53XU2, Q9UNV3, B3KT15,
UniProt Related Accession:Q9UNM6
Molecular Weight:43kDa
NCBI Full Name:26S proteasome non-ATPase regulatory subunit 13
NCBI Synonym Full Names:proteasome 26S subunit, non-ATPase 13
NCBI Official Symbol:PSMD13��
NCBI Official Synonym Symbols:S11; Rpn9; p40.5; HSPC027��
NCBI Protein Information:26S proteasome non-ATPase regulatory subunit 13
UniProt Protein Name:26S proteasome non-ATPase regulatory subunit 13
UniProt Synonym Protein Names:26S proteasome regulatory subunit RPN9; 26S proteasome regulatory subunit S11; 26S proteasome regulatory subunit p40.5
Protein Family:26S proteasome non-ATPase regulatory
UniProt Gene Name:PSMD13��
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