Enzymes Recombinant Proteins

Human PSMB8 Recombinant Protein (RPPB2211)

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SKU:
RPPB2211
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P28062
Research Area:
Enzymes

Description

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Product Name:Human PSMB8 Recombinant Protein
Product Code:RPPB2211
Size:20µg
Species:Human
Target:PSMB8
Synonyms:D6S216, D6S216E, LMP7, MGC1491, PSMB5i , Low molecular mass protein 7 Macropain subunit C13, Multicatalytic endopeptidase complex subunit C13,Proteasome component C13, Proteasome subunit beta-5i, Really interesting new gene 10 protein.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:PSMB8 protein solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMTTTLA FKFQHGVIAA VDSRASAGSY ISALRVNKVI EINPYLLGTM SGCAADCQYW ERLLAKECRL YYLRNGERIS VSAASKLLSN MMCQYRGMGL SMGSMICGWD KKGPGLYYVD EHGTRLSGNM FSTGSGNTYA YGVMDSGYRP NLSPEEAYDL GRRAIAYATH RDSYSGGVVN MYHMKEDGWV KVESTDVSDL LHQYREANQ

Proteasome Subunit Beta Type 8 (PSMB8) is a member of the peptidase T1B family. The proteasome is a multicatalytic proteinase complex with well-organized ring-shaped 20S core structure. Proteasomes are spread through eukaryotic cells at a high concentration. PSMB8 is mainly expressed in immature dendritic cells. vital function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.PSMB8 is positioned in the class II region of the MHC (main histocompatibility complex). PSMB8 plays as a significant factor of INFG-induced sensitivity and in apoptosis by degradation of the apoptotic inhibitor MCL1.

PSMB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 229 amino acids (73-276 a.a.) and having a molecular mass of 25.4kDa. PSMB8 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMB8: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternatively spliced isoforms have been identified; both isoforms are processed to yield the same mature subunit.
UniProt Protein Details:

Protein type:Protease; EC 3.4.25.1; Proteasome complex

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytosol

Molecular Function:threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; fat cell differentiation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Disease: Autoinflammation, Lipodystrophy, And Dermatosis Syndrome

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
UniProt Code:P28062
NCBI GenInfo Identifier:334302881
NCBI Gene ID:5696
NCBI Accession:P28062.3
UniProt Secondary Accession:P28062,P28063, P28064,
UniProt Related Accession:P28062
Molecular Weight:
NCBI Full Name:Proteasome subunit beta type-8
NCBI Synonym Full Names:proteasome 20S subunit beta 8
NCBI Official Symbol:PSMB8��
NCBI Official Synonym Symbols:JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E��
NCBI Protein Information:proteasome subunit beta type-8
UniProt Protein Name:Proteasome subunit beta type-8
UniProt Synonym Protein Names:Low molecular mass protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; Proteasome component C13; Proteasome subunit beta-5i; Really interesting new gene 10 protein
UniProt Gene Name:PSMB8��
UniProt Entry Name:PSB8_HUMAN
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