Enzymes Recombinant Proteins

Human PSMB6 Recombinant Protein (RPPB2209)

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SKU:
RPPB2209
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P28072
Research Area:
Enzymes

Description

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Product Name:Human PSMB6 Recombinant Protein
Product Code:RPPB2209
Size:10µg
Species:Human
Target:PSMB6
Synonyms:Proteasome subunit beta type-6, Macropain delta chain, Multicatalytic endopeptidase complex delta chain, Proteasome delta chain, Proteasome subunit Y, PSMB6, LMPY, Y, DELTA.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PSMB6 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.15M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTTIMAVQFD GGVVLGADSR TTTGSYIANR VTDKLTPIHD RIFCCRSGSA ADTQAVADAV TYQLGFHSIE LNEPPLVHTA ASLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA LALAMERDGS SGGVIRLAAI AESGVERQVL LGDQIPKFAV ATLPPA

PSMB6 belongs to the proteasome B-type family (T1B family) which is a 20S core beta subunit in the proteasome. The PSMB6 is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with an extremely ordered ring-shaped 20S core structure. The core structure is comprised of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.

PSMB6 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 226 amino acids (35-239) and having a molecular mass of 24.2kDa.PSMB6 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMB6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the peptidyl glutamyl-like activity. May catalyze basal processing of intracellular antigens. Belongs to the peptidase T1B family.
UniProt Protein Details:

Protein type:Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: nucleoplasm; proteasome complex; Golgi apparatus; proteasome core complex; cytoplasm; nucleus; cytosol

Molecular Function:threonine endopeptidase activity; endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:P28072
NCBI GenInfo Identifier:20532407
NCBI Gene ID:5694
NCBI Accession:P28072.4
UniProt Related Accession:P28072
Molecular Weight:
NCBI Full Name:Proteasome subunit beta type-6
NCBI Synonym Full Names:proteasome subunit beta 6
NCBI Official Symbol:PSMB6��
NCBI Official Synonym Symbols:Y; LMPY; DELTA��
NCBI Protein Information:proteasome subunit beta type-6
UniProt Protein Name:Proteasome subunit beta type-6
UniProt Synonym Protein Names:Macropain delta chain; Multicatalytic endopeptidase complex delta chain; Proteasome delta chain; Proteasome subunit Y
Protein Family:Proteasome
UniProt Gene Name:PSMB6��
UniProt Entry Name:PSB6_HUMAN
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