Enzymes Recombinant Proteins

Human PSMB4 Recombinant Protein (RPPB2207)

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SKU:
RPPB2207
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P28070
Research Area:
Enzymes

Description

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Product Name:Human PSMB4 Recombinant Protein
Product Code:RPPB2207
Size:10µg
Species:Human
Target:PSMB4
Synonyms:Proteasome (prosome, macropain) subunit beta type 4, PROS26, HN3, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome chain 3, 26 kDa prosomal protein, HsN3, PROS-26, hsBPROS26, EC 3.4.25.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PSMB4 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 1mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE

PSMB4 is a member of the peptidase T1B family. The 20S Proteasome chamber holds structural alpha subunits and predominantly catalytic beta subunits. The two external rings in the proteasome contain seven alpha subunits each and the two internal rings contain of seven beta subunits each. PSMB4 is a beta subunit of the 20S Proteasome that is spread all over the eukaryotic cells in large quantities and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.

PSMB4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 240 amino acids (46-264 a.a.) and having a molecular mass of 26.6kDa.PSMB4 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PSMB4: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway.
UniProt Protein Details:

Protein type:Proteasome complex; Cell cycle regulation; EC 3.4.25.1; Protease; Cytoskeletal

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol; nucleus

Molecular Function:threonine endopeptidase activity; lipopolysaccharide binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; negative regulation of inflammatory response to antigenic stimulus; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle

NCBI Summary:The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008]
UniProt Code:P28070
NCBI GenInfo Identifier:116242733
NCBI Gene ID:5692
NCBI Accession:P28070.4
UniProt Secondary Accession:P28070,P31148, Q5SZS5, Q6IBI4, Q969L6, B2R9L3,
UniProt Related Accession:P28070
Molecular Weight:264
NCBI Full Name:Proteasome subunit beta type-4
NCBI Synonym Full Names:proteasome (prosome, macropain) subunit, beta type, 4
NCBI Official Symbol:PSMB4��
NCBI Official Synonym Symbols:HN3; HsN3; PROS26; PROS-26��
NCBI Protein Information:proteasome subunit beta type-4; hsBPROS26; proteasome chain 3; macropain beta chain; proteasome beta chain; 26 kDa prosomal protein; proteasome subunit HsN3; proteasome subunit, beta type, 4; multicatalytic endopeptidase complex beta chain
UniProt Protein Name:Proteasome subunit beta type-4
UniProt Synonym Protein Names:26 kDa prosomal protein; HsBPROS26; PROS-26; Macropain beta chain; Multicatalytic endopeptidase complex beta chain; Proteasome beta chain; Proteasome chain 3; HsN3
Protein Family:Proteasome
UniProt Gene Name:PSMB4��
UniProt Entry Name:PSB4_HUMAN
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