Enzymes Recombinant Proteins

Human PRKACA Recombinant Protein (RPPB2631)

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RPPB2631

Description

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Product Name:Human PRKACA Recombinant Protein
Product Code:RPPB2631
Size:5µg
Species:Human
Target:PRKACA
Synonyms:cAMP-dependent protein kinase alpha-catalytic subunit, EC 2.7.11.11, PKA C-alpha, PKACA, PRKACA, MGC48865, MGC102831.
Source:Escherichia Coli
Formulation:PKA catalytic subunit a is supplied in a buffer containing 20mM MOPS pH7, 150mM NaCl, 1mM DTT, 1mM EDTA and 50% Glycerin.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL GTGSFGRVML VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F

cAMP-dependent PKA is an ubiquitous serine/threonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catalytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA catalytic subunit a is a 41kDa protein. The a-isoform is the predominant form with a broad tissue distribution and can be used for in vitro enzymological studies of neural and hormonal signal transduction or to phosphorylate target proteins in vivo including Ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism.

UniProt Protein Function:PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; AGC group; PKA family

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: centrosome; membrane; mitochondrion; plasma membrane; cytosol; nucleus; neuromuscular junction; AMP-activated protein kinase complex; cAMP-dependent protein kinase complex

Molecular Function:protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; water transport; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; regulation of synaptic transmission; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytosolic calcium ion homeostasis; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; neural tube closure; carbohydrate metabolic process; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; regulation of insulin secretion

NCBI Summary:cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:P17612
NCBI GenInfo Identifier:125205
NCBI Gene ID:5566
NCBI Accession:P17612.2
UniProt Secondary Accession:P17612,Q32P54, Q9H2Y0, Q9NRB4, Q9NRH9,
UniProt Related Accession:P17612
Molecular Weight:39,953 Da
NCBI Full Name:cAMP-dependent protein kinase catalytic subunit alpha
NCBI Synonym Full Names:protein kinase, cAMP-dependent, catalytic, alpha
NCBI Official Symbol:PRKACA��
NCBI Official Synonym Symbols:PKACA��
NCBI Protein Information:cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; protein kinase A catalytic subunit; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1
UniProt Protein Name:cAMP-dependent protein kinase catalytic subunit alpha
Protein Family:cAMP-dependent protein kinase
UniProt Gene Name:PRKACA��
UniProt Entry Name:KAPCA_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P17612
Research Area:
Enzymes
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