Enzymes Recombinant Proteins

Human PPP2R1A Recombinant Protein (RPPB2169)

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SKU:
RPPB2169
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P30153
Research Area:
Enzymes

Description

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Product Name:Human PPP2R1A Recombinant Protein
Product Code:RPPB2169
Size:20µg
Species:Human
Target:PPP2R1A
Synonyms:PPP2R1A,Protein Phosphatase 2, Regulatory Subunit A, Alpha, Protein Phosphatase 2(Formerly 2A), Regulatory Subunit A (PR 65), Alpha Isoform, PP2A Subunit AIsoform PR65-Alpha, PP2A Subunit A Isoform R1-Alpha, Serine/Threonine ProteinPhosphatase 2A, 65 KDa Regulatory Subunit A, Alpha Isoform, Serine/Threonine-ProteinPhosphatase 2A, 65 KDa Regulatory Subunit A Alpha Isoform, Protein Phosphatase2 (Formerly 2A), Regulatory Subunit A, Alpha Isoform, Medium TumorAntigen-Associated 61 KDA Protein, Medium Tumor Antigen-Associated 61 KDaProtein, 65kDa Regulatory Subunit A, Protein Phosphatase 2A, ProteinPhosphatase 2, Regulatory Subunit A, Alpha Isoform, PP2A-Aalpha, PP2AAALPHA, PR65A.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:PPP2R1A protein solution (1mg/ml) containing PhosphateBuffered Saline (pH7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greaterthan 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAAADGD DSLYPIAVLI DELRNEDVQL RLNSIKKLST IALALGVERT RSELLPFLTD TIYDEDEVLL ALAEQLGTFT TLVGGPEYVH CLLPPLESLA TVEETVVRDK AVESLRAISH EHSPSDLEAH FVPLVKRLAG GDWFTSRTSA CGLFSVCYPR VSSAVKAELR QYFRNLCSDD TPMVRRAAAS KLGEFAKVLE LDNVKSEIIP MFSNLASDEQ DSVRLLAVEA CVNIAQLLPQ EDLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDANQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA

Protein Phosphatase 2, Regulatory Subunit AAlpha, also known as PPP2R1A is part of the PPP family of phosphatases,which regulates a diversity of vital cellular processes. PPP2R1A subunit, PR65,functions as a scaffolding molecule which coordinates the assembly of thecatalytic subunit and a variable regulatory B subunit. PPP2R1A is essential forproper chromosome segregation as well as for centromeric localization of SGOL1in mitosis. Furthermore, PPP2R1A phosphorylation is an in vivo mechanism whichregulates the PP2A signaling complex as well as increasing the PP2A activity inheart failure.

PPP2R1A Human Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 612 amino acids (1-589 a.a) andhaving a molecular mass of 67.7kDa.PPP2R1A is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

UniProt Protein Function:PPP2R1A: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Required for proper chromosome segregation and for centromeric localization of SGOL1 in mitosis. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGOL1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Belongs to the phosphatase 2A regulatory subunit A family.
UniProt Protein Details:

Protein type:Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: microtubule cytoskeleton; protein phosphatase type 2A complex; mitochondrion; membrane; nucleus; cytosol; chromosome, pericentric region

Molecular Function:protein binding; protein heterodimerization activity; protein phosphatase type 2A regulator activity; protein serine/threonine phosphatase activity; antigen binding

Biological Process: regulation of cell adhesion; second-messenger-mediated signaling; mitotic nuclear envelope reassembly; fibroblast growth factor receptor signaling pathway; apoptosis; organelle organization and biogenesis; RNA splicing; regulation of Wnt receptor signaling pathway; protein amino acid dephosphorylation; chromosome segregation; response to organic substance; regulation of transcription, DNA-dependent; regulation of growth; regulation of cell differentiation; mRNA catabolic process, nonsense-mediated decay; negative regulation of tyrosine phosphorylation of Stat3 protein; protein complex assembly; gene expression; mitotic cell cycle; negative regulation of cell growth; G2/M transition of mitotic cell cycle; ceramide metabolic process; inactivation of MAPK activity; regulation of DNA replication

Disease: Mental Retardation, Autosomal Dominant 36

NCBI Summary:This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
UniProt Code:P30153
NCBI GenInfo Identifier:143811355
NCBI Gene ID:5518
NCBI Accession:P30153.4
UniProt Secondary Accession:P30153,Q13773, Q6ICQ3, Q96DH3,
UniProt Related Accession:P30153
Molecular Weight:589
NCBI Full Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
NCBI Synonym Full Names:protein phosphatase 2, regulatory subunit A, alpha
NCBI Official Symbol:PPP2R1A��
NCBI Official Synonym Symbols:PR65A; PP2AAALPHA; PP2A-Aalpha��
NCBI Protein Information:serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2A subunit A isoform R1-alpha; PP2A subunit A isoform PR65-alpha; medium tumor antigen-associated 61 KDA protein; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
UniProt Synonym Protein Names:Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha
UniProt Gene Name:PPP2R1A��
UniProt Entry Name:2AAA_HUMAN
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