Enzymes Recombinant Proteins

Human PPM1G Recombinant Protein (RPPB2160)

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SKU:
RPPB2160
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O15355
Research Area:
Enzymes

Description

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Product Name:Human PPM1G Recombinant Protein
Product Code:RPPB2160
Size:10µg
Species:Human
Target:PPM1G
Synonyms:Protein Phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2C GAMMA, EC 3.1.3.16, Protein phosphatase 2C isoform gamma, PP2C-gamma, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase 1C, PPM1G, PPM1C.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The PPM1G solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl, 0.1mM PMSF and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD

PPM1G is part of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1G is accountable for the dephosphorylation of Pre-mRNA splicing factors, an important factor for the formation of functional spliceosome. PPM1G regulates cell cycle progression. PPM1G mediates histone dephosphorylation/exchange in response to DNA damage or checkpoint recovery in higher eukaryotes. The degradation of p21/WAF1 induced by PPM1G is mediated in a proteasome-dependent manner. Protein phosphatase 1G regulates assembly and function of the beta-catenin degradation complex.

PPM1G Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 566 amino acids (1-546) and having a molecular mass of 61.4 kDa. The PPM1G is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
UniProt Protein Details:

Protein type:Spliceosome; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation; cell cycle arrest

NCBI Summary:The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
UniProt Code:O15355
NCBI GenInfo Identifier:3122589
NCBI Gene ID:5496
NCBI Accession:O15355.1
UniProt Related Accession:O15355
Molecular Weight:
NCBI Full Name:Protein phosphatase 1G
NCBI Synonym Full Names:protein phosphatase, Mg2+/Mn2+ dependent 1G
NCBI Official Symbol:PPM1G��
NCBI Official Synonym Symbols:PP2CG; PPP2CG; PP2CGAMMA��
NCBI Protein Information:protein phosphatase 1G
UniProt Protein Name:Protein phosphatase 1G
UniProt Synonym Protein Names:Protein phosphatase 1C; Protein phosphatase 2C isoform gamma; PP2C-gamma; Protein phosphatase magnesium-dependent 1 gamma
Protein Family:Protein phosphatase
UniProt Gene Name:PPM1G��
UniProt Entry Name:PPM1G_HUMAN
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