Enzymes Recombinant Proteins

Human PPM1D Recombinant Protein (RPPB5825)

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SKU:
RPPB5825
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O15297
Research Area:
Enzymes

Description

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Product Name:Human PPM1D Recombinant Protein
Product Code:RPPB5825
Size:10µg
Species:Human
Target:PPM1D
Synonyms:Protein phosphatase 1D magnesium-dependent, delta isoform, PPM1D, Protein Phosphatase 1D, PP2C-DELTA, WIP1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:PPM1D protein solution (0.5mg/ml) containing 20mM Tris-HCl (pH8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MVAFFAVCDG HGGREAAQFA REHFWGFIKK QKGFTSSEPA KVCAAIRKGF LACHLAMWKK LAEWPKTMTG LPSTSGTTAS VVIIRGMKMY VAHVGDSGVV LGIQDDPKDD FVRAVEVTQD HKPELPKERE RIEGLGGSVM NKSGVNRVVW KRPRLTHNGP VRRSTVIDQI PFLAVARALG DLWSYDFFSG EFVVSPEPDT SVHTLDPQKH KYIILGSDGL WNMIPPQDAI SMCQDQEEKK YLMGEHGQSC AKMLVNRALG RWRQRMLRAD NTSAIVICI

ProteinPhosphatase 1D (PPM1D) belongs to the PP2C family of Ser/Thr proteinphosphatases whose members are negative regulators of cell stress responsepathways. PPM1D is induced by tumor suppressor protein TP53/p53 in response to multipleenvironmental stresses. PPM1D is a negative regulator of the activity of p38MAP kinase through which it reduces the phosphorylation of p53 and suppressesp53-mediated transcription and apoptosis. PPM1D is located in a chromosomal areawhich enlarges in breast cancer.

PPM1D Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 299 amino acids (98-375a.a.) and having a molecular mass of 33.2kDa.PPM1D is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PPM1D: a magnesium-dependent Ser/Thr phosphatase. A p53-induced phosphatase that interacts with the nuclear isoform of uracil DNA glycosylase, UNG2, and suppresses base excision repair. Might contribute to growth inhibitory pathways activated in response to DNA damage in a p53-dependent manner.
UniProt Protein Details:

Protein type:Cell cycle regulation; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 17q23.2

Cellular Component: nucleus

Molecular Function:mitogen-activated protein kinase binding; protein binding; protein serine/threonine phosphatase activity

Biological Process: negative regulation of cell proliferation; protein amino acid dephosphorylation; response to radiation

Disease: Breast Cancer

NCBI Summary:The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]
UniProt Code:O15297
NCBI GenInfo Identifier:10720176
NCBI Gene ID:8493
NCBI Accession:O15297.1
UniProt Secondary Accession:O15297,Q53XP4, Q6P991, Q8IVR6,
UniProt Related Accession:O15297
Molecular Weight:47,211 Da
NCBI Full Name:Protein phosphatase 1D
NCBI Synonym Full Names:protein phosphatase, Mg2+/Mn2+ dependent 1D
NCBI Official Symbol:PPM1D��
NCBI Official Synonym Symbols:WIP1; PP2C-DELTA��
NCBI Protein Information:protein phosphatase 1D
UniProt Protein Name:Protein phosphatase 1D
UniProt Synonym Protein Names:Protein phosphatase 2C isoform delta; PP2C-delta; Protein phosphatase magnesium-dependent 1 delta; p53-induced protein phosphatase 1
Protein Family:Protein phosphatase
UniProt Gene Name:PPM1D��
UniProt Entry Name:PPM1D_HUMAN
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