Enzymes Recombinant Proteins

Human PPCS Recombinant Protein (RPPB2152)

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RPPB2152

Description

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Product Name:Human PPCS Recombinant Protein
Product Code:RPPB2152
Size:20µg
Species:Human
Target:PPCS
Synonyms:Phosphopantothenate--cysteine ligase, Phosphopantothenoylcysteine synthetase, PPC synthetase, PPCS, COAB, RP11-163G10.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:PPCS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Stability:PPCS Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N

Phosphopantothenate-cysteine ligase (PPCS) catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is a vital universal pathway in prokaryotes and eukaryotes. PPCS, being one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.

PPCS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 331 amino acids (1-311 a.a.) and having a molecular mass of 36.1kDa.PPCS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:PPCS: Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'- phosphopantothenate to form 4-phosphopantothenoylcysteine. Belongs to the PPC synthetase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 6.3.2.5; Ligase

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cytosol

Molecular Function:phosphopantothenate--cysteine ligase activity

Biological Process: coenzyme A biosynthetic process; coenzyme biosynthetic process

NCBI Summary:Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9HAB8
NCBI GenInfo Identifier:47117318
NCBI Gene ID:79717
NCBI Accession:Q9HAB8.2
UniProt Secondary Accession:Q9HAB8,Q3KQT2, Q5VVM0,
UniProt Related Accession:Q9HAB8
Molecular Weight:34kDa
NCBI Full Name:Phosphopantothenate--cysteine ligase
NCBI Synonym Full Names:phosphopantothenoylcysteine synthetase
NCBI Official Symbol:PPCS��
NCBI Protein Information:phosphopantothenate--cysteine ligase
UniProt Protein Name:Phosphopantothenate--cysteine ligase
UniProt Synonym Protein Names:Phosphopantothenoylcysteine synthetase; PPC synthetase
Protein Family:Phosphopantothenate--cysteine ligase
UniProt Gene Name:PPCS��
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9HAB8
Research Area:
Enzymes
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