Human POU2AF1 Recombinant Protein (RPPB4281)

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SKU:
RPPB4281
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q16633

Description

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Product Name:Human POU2AF1 Recombinant Protein
Product Code:RPPB4281
Size:10µg
Species:Human
Target:POU2AF1
Synonyms:POU domain class 2 associating factor 1, BOB-1, OCA-B, B-cell-specific coactivator OBF-1, OCT-binding factor 1.
Source:Escherichia Coli
Physical Appearance:POU2AF1 is supplied as a sterile filtered clear solution.
Formulation:POU2AF1 protein (0.5mg/ml) is supplied in 20mM Tris-HCL, pH-8 and 20% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE DSDAYALNHT LSVEGF

POU2AF1 is a lymphocyte specific transcription coactivator protein. POU2AF1 cooperates only with the Oct1/2 proteins using sub domains in the POU domain of the Oct1/2 proteins, increasing their transcriptional efficiency. Even though POU2AF1 have no basic ability to bind DNA, it links firmly with the octomer motif in the presence of Oct1 and Oct2. POU2AF1 is expressed at maximum levels in spleen and peripheral blood leukocytes.

POU2AF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 276 amino acids (1-256) and having a molecular mass of 29.6 kDa.The POU2AF1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.
UniProt Protein Details:

Protein type:Oncoprotein; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: nucleus

Molecular Function:protein binding; DNA binding; transcription coactivator activity; transcription cofactor activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; humoral immune response

UniProt Code:Q16633
NCBI GenInfo Identifier:2833276
NCBI Gene ID:5450
NCBI Accession:Q16633.1
UniProt Secondary Accession:Q16633,Q14983, B2R8Z9,
UniProt Related Accession:Q16633,AAB34937
Molecular Weight:256
NCBI Full Name:POU domain class 2-associating factor 1
NCBI Synonym Full Names:POU class 2 associating factor 1
NCBI Official Symbol:POU2AF1��
NCBI Official Synonym Symbols:BOB1; OBF1; OCAB; OBF-1��
NCBI Protein Information:POU domain class 2-associating factor 1; BOB-1; OCA-B; OCT-binding factor 1; B-cell-specific coactivator OBF-1; POU domain, class 2, associating factor 1
UniProt Protein Name:POU domain class 2-associating factor 1
UniProt Synonym Protein Names:B-cell-specific coactivator OBF-1; BOB-1; OCA-B; OCT-binding factor 1
Protein Family:POU domain class 2-associating factor
UniProt Gene Name:POU2AF1��
UniProt Entry Name:OBF1_HUMAN
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