Human POR Recombinant Protein (RPPB2146)
- SKU:
- RPPB2146
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P16435
Description
| Product Name: | Human POR Recombinant Protein |
| Product Code: | RPPB2146 |
| Size: | 10µg |
| Species: | Human |
| Target: | POR |
| Synonyms: | P450 (Cytochrome) Oxidoreductase, EC 1.6.2.4, CYPOR, P450R,CPR, NADPH-Dependent Cytochrome P450 Reductase, NADPH--Cytochrome P450Reductase, NADPH--cytochrome P450 reductase. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | POR protein solution (0.25mg/ml) contains Phosphatebuffer saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MINMGDSHVDTSSTVSEAVA EEVSLFSMTD MILFSLIVGL LTYWFLFRKK KEEVPEFTKI QTLTSSVRES SFVEKMKKTGRNIIVFYGSQ TGTAEEFANR LSKDAHRYGM RGMSADPEEY DLADLSSLPE IDNALVVFCM ATYGEGDPTDNAQDFYDWLQ ETDVDLSGVK FAVFGLGNKT YEHFNAMGKY VDKRLEQLGA QRIFELGLGD DDGNLEEDFITWREQFWLAV CEHFGVEATG EESSIRQYEL VVHTDIDAAK VYMGEMGRLK SYENQKPPFD AKNPFLAAVTTNRKLNQGTE RHLMHLELDI SDSKIRYESG DHVAVYPAND SALVNQLGKI LGADLDVVMS LNNLDEESNKKHPFPCPTSY RTALTYYLDI TNPPRTNVLY ELAQYASEPS EQELLRKMAS SSGEGKELYL SWVVEARRHILAILQDCPSL RPPIDHLCEL LPRLQARYYS IASSSKVHPN SVHICAVVVE YETKAGRINK GVATNWLRAKEPVGENGGRA LVPMFVRKSQ FRLPFKATTP VIMVGPGTGV APFIGFIQER AWLRQQGKEV GETLLYYGCRRSDEDYLYRE ELAQFHRDGA LTQLNVAFSR EQSHKVYVQH LLKQDREHLW KLIEGGAHIY VCGDARNMARDVQNTFYDIV AELGAMEHAQ AVDYIKKLMT KGRYSLDVWS HHHHHH |
P450 Oxidoreductase, also known as POR is a flavoprotein which contributeselectrons to all microsomal P450 enzymes. POR is localized to the endoplasmic reticulum, where it is also capableof transfering electrons to heme oxygenase as well as cytochrome b5. POR isstructurally related to two separate flavoprotein families; first one is ferredoxinnucleotide reductase and the second flavodoxin.
POR produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 686 amino acids (1-680a.a.) andhaving a molecular mass of 77.9kDa. POR is expressed with a 6 amino acid His tag atC-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome. |
| UniProt Protein Details: | Protein type:EC 1.6.2.4; Endoplasmic reticulum; Mitochondrial; Oxidoreductase Chromosomal Location of Human Ortholog: 7q11.2 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane Molecular Function:[methionine synthase] reductase activity; NADPH-hemoprotein reductase activity; protein binding Biological Process: positive regulation of monooxygenase activity; xenobiotic metabolic process Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency |
| NCBI Summary: | This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P16435 |
| NCBI GenInfo Identifier: | 2851393 |
| NCBI Gene ID: | 5447 |
| NCBI Accession: | P16435.2 |
| UniProt Related Accession: | P16435 |
| Molecular Weight: | |
| NCBI Full Name: | NADPH--cytochrome P450 reductase |
| NCBI Synonym Full Names: | cytochrome p450 oxidoreductase |
| NCBI Official Symbol: | POR |
| NCBI Official Synonym Symbols: | CPR; CYPOR; P450R |
| NCBI Protein Information: | NADPH--cytochrome P450 reductase |
| UniProt Protein Name: | NADPH--cytochrome P450 reductase |
| Protein Family: | Pyruvate synthase |
| UniProt Gene Name: | POR |
| UniProt Entry Name: | NCPR_HUMAN |
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