Human POLR2J Recombinant Protein (RPPB4275)
- SKU:
- RPPB4275
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P52435
Description
| Product Name: | Human POLR2J Recombinant Protein |
| Product Code: | RPPB4275 |
| Size: | 10µg |
| Species: | Human |
| Target: | POLR2J |
| Synonyms: | DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II subunit B11-a, RPB11a, DNA-directed RNA polymerase II subunit J-1, RNA polymerase II 13.3 kDa subunit, POLR2J, POLR2J1, RPB11, RPB11m, hRPB14. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | POLR2J protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK KITINKDTKV PNACLFTINK EDHTLGNIIK SQLLKDPQVL FAGYKVPHPL EHKIIIRVQT TPDYSPQEAF TNAITDLISE LSLLEERFRV AIKDKQEGIE |
Polymerase (RNA) II (DNA directed) Polypeptide J (POLR2J) is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The POLR2J protein occurs as a heterodimer with another polymerase subunit; together they develop a core subassembly unit of the polymerase. POLR2J is ubiquitously expressed, having a high expression in the heart and skeletal muscle.
POLR2J Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 140 amino acids (1-117 a.a) and having a molecular mass of 15.7kDa.POLR2J is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | POLR2J: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. |
| UniProt Protein Details: | Protein type:Transferase; DNA-binding; RNA splicing; RNA processing Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: DNA-directed RNA polymerase II, core complex; nucleoplasm; nucleus Molecular Function:DNA-directed RNA polymerase activity; LRR domain binding; protein binding Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA capping; nuclear mRNA splicing, via spliceosome; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; RNA-mediated gene silencing; snRNA transcription from RNA polymerase II promoter; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair |
| NCBI Summary: | This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P52435 |
| NCBI GenInfo Identifier: | 1710661 |
| NCBI Gene ID: | 5439 |
| NCBI Accession: | P52435.1 |
| UniProt Secondary Accession: | P52435,O43375, A5D6V8, |
| UniProt Related Accession: | P52435 |
| Molecular Weight: | 13,293 Da |
| NCBI Full Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| NCBI Synonym Full Names: | RNA polymerase II subunit J |
| NCBI Official Symbol: | POLR2J�� |
| NCBI Official Synonym Symbols: | RPB11; RPB11A; RPB11m; hRPB14; POLR2J1�� |
| NCBI Protein Information: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Protein Name: | DNA-directed RNA polymerase II subunit RPB11-a |
| UniProt Synonym Protein Names: | DNA-directed RNA polymerase II subunit J-1; RNA polymerase II 13.3 kDa subunit |
| Protein Family: | DNA-directed RNA polymerase |
| UniProt Gene Name: | POLR2J�� |
| UniProt Entry Name: | RPB11_HUMAN |
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