Enzymes Recombinant Proteins

Human POLR2F Recombinant Protein (RPPB2138)

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SKU:
RPPB2138
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P61218
Research Area:
Enzymes

Description

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Product Name:Human POLR2F Recombinant Protein
Product Code:RPPB2138
Size:20µg
Species:Human
Target:POLR2F
Synonyms:Polymerase (RNA) II (DNA Directed) Polypeptide F, DNA Directed RNA Polymerase II 14.4 Kda Polypeptide, POLRF, RPABC14.4, RPB14.4, RPC15, DNA-Directed RNA Polymerase II Subunit F, DNA-Directed RNA Polymerases I, II, And III 14.4 KDa Polypeptide, RNA Polymerases I, II, And III Subunit ABC2, RPB6 Homolog, HRBP14.4, RPABC2, RPB6, DNA-Directed RNA Polymerases I, II, And III Subunit RPABC2, RNA Polymerase II Subunit 14.4 KD, POLR2F.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The POLR2F solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSDNEDN FDGDDFDDVE EDEGLDDLEN AEEEGQENVE ILPSGERPQA NQKRITTPYM TKYERARVLG TRALQIAMCA PVMVELEGET DPLLIAMKEL KARKIPIIIR RYLPDGSYED WGVDELIITD

Polymerase II Polypeptide F (POLR2F) is the 6th largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, which is also shared by the other two DNA-directed RNA polymerases. In yeast, POLR2F, in combination with at least 2 other subunits, forms a structure which stabilizes the transcribing polymerase on the DNA template. DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA via the 4 ribonucleoside triphosphates as substrates. Pol II is the principal element of the basal RNA polymerase II transcription apparatus. Pols are comprised of mobile elements which move close to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and along with parts of RPB1 and RPB2 constructs a compartment to which the RPB4-RPB7 subcomplex attaches.

POLR2F Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.9kDa (Molecular size on SDS-PAGE will appear higher).POLR2F is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:POLR2F: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and togther with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds. Belongs to the archaeal RpoK/eukaryotic RPB6 RNA polymerase subunit family.
UniProt Protein Details:

Protein type:RNA processing; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transcription initiation complex; Transferase; EC 2.7.7.6

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; nucleolus; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex

Molecular Function:DNA binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; positive regulation of viral transcription; somatic stem cell maintenance; RNA splicing; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter

NCBI Summary:This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:P61218
NCBI GenInfo Identifier:47117761
NCBI Gene ID:5435
NCBI Accession:P61218.1
UniProt Secondary Accession:P61218,P41584, Q6IAY3,
UniProt Related Accession:P61218
Molecular Weight:127
NCBI Full Name:DNA-directed RNA polymerases I, II, and III subunit RPABC2
NCBI Synonym Full Names:polymerase (RNA) II (DNA directed) polypeptide F
NCBI Official Symbol:POLR2F��
NCBI Official Synonym Symbols:RPB6; POLRF; RPC15; RPABC2; RPB14.4; HRBP14.4; RPABC14.4��
NCBI Protein Information:DNA-directed RNA polymerases I, II, and III subunit RPABC2; RNA Polymerase II subunit 14.4 kD; DNA-directed RNA polymerase II subunit F; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide
UniProt Protein Name:DNA-directed RNA polymerases I, II, and III subunit RPABC2
UniProt Synonym Protein Names:DNA-directed RNA polymerase II subunit F; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide; RPABC14.4; RPB14.4; RPB6 homolog; RPC15
UniProt Gene Name:POLR2F��
UniProt Entry Name:RPAB2_HUMAN
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