Enzymes Recombinant Proteins

Human POGLUT1 Recombinant Protein (RPPB2133)

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SKU:
RPPB2133
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8NBL1
Research Area:
Enzymes

Description

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Product Name:Human POGLUT1 Recombinant Protein
Product Code:RPPB2133
Size:5µg
Species:Human
Target:POGLUT1
Synonyms:POGLUT1, C3orf9, CLP46, hCLP46, KDELCL1, KTELC1, ProteinO-glucosyltransferase 1, CAP10-like 46 kDa protein, KTEL motif-containingprotein 1, Myelodysplastic syndromes relative protein, O-glucosyltransferaseRumi homolog, hRumi, Protein O-xylosyltransferase, MDSRP.
Source:Sf9 Insect cells
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The POGLUT1 solution (0.25mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:RQKESGSKWKVFIDQINRSL ENYEPCSSQN CSCYHGVIEE DLTPFRGGIS RKMMAEVVRR KLGTHYQITK NRLYRENDCMFPSRCSGVEH FILEVIGRLP DMEMVINVRD YPQVPKWMEP AIPVFSFSKT SEYHDIMYPA WTFWEGGPAVWPIYPTGLGR WDLFREDLVR SAAQWPWKKK NSTAYFRGSR TSPERDPLIL LSRKNPKLVD AEYTKNQAWKSMKDTLGKPA AKDVHLVDHC KYKYLFNFRG VAASFRFKHL FLCGSLVFHV GDEWLEFFYP QLKPWVHYIPVKTDLSNVQE LLQFVKANDD VAQEIAERGS QFIRNHLQMD DITCYWENLL SEYSKFLSYN VTRRKGYDQIIPKMLKTELL EHHHHHH

POGLUT1 is a homologue of Rumi fromDrosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase which catalyzesthe transfer of glucose and xylose from UDP-glucose and UDP-xylose,respectively, to EGF repeats on the consensus sequence C-X-S-X-P-C. POGLUT1 positivelyregulates Notch signaling without affecting Notch ligand binding.

POGLUT1 Human Recombinant produced in in Sf9 Baculoviruscells is a single, non-glycosylated polypeptide chain containing 377 aminoacids (24-392a.a) and having a molecular mass of 44.5kDa (Migrates at 40-57kDaon SDS-PAGE under reducing conditions).POGLUT1 is fused to a 8 amino acid His-tag at C-Terminusand purified by proprietary chromatographic techniques.

UniProt Protein Function:KTELC1: UDP-glucosyltransferase. Belongs to the glycosyltransferase 90 family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide; EC 2.4.2.26

Chromosomal Location of Human Ortholog: 3q13.33

Cellular Component: endoplasmic reticulum lumen

Molecular Function:glucosyltransferase activity; UDP-glucosyltransferase activity; UDP-xylosyltransferase activity

Biological Process: glycolipid metabolic process; positive regulation of Notch signaling pathway; protein amino acid O-linked glycosylation

Disease: Dowling-degos Disease 4

NCBI Summary:This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt Code:Q8NBL1
NCBI GenInfo Identifier:74730148
NCBI Gene ID:56983
NCBI Accession:Q8NBL1.1
UniProt Secondary Accession:Q8NBL1,Q53GJ4, Q8N2T1, B2RD13,
UniProt Related Accession:Q8NBL1
Molecular Weight:46,189 Da
NCBI Full Name:Protein O-glucosyltransferase 1
NCBI Synonym Full Names:protein O-glucosyltransferase 1
NCBI Official Symbol:POGLUT1��
NCBI Official Synonym Symbols:Rumi; CLP46; MDSRP; C3orf9; KTELC1; MDS010; hCLP46; KDELCL1��
NCBI Protein Information:protein O-glucosyltransferase 1
UniProt Protein Name:Protein O-glucosyltransferase 1
UniProt Synonym Protein Names:CAP10-like 46 kDa protein; hCLP46; KTEL motif-containing protein 1; Myelodysplastic syndromes relative protein; O-glucosyltransferase Rumi homolog; hRumi; Protein O-xylosyltransferase (EC:2.4.2.26)
Protein Family:Protein O-glucosyltransferase
UniProt Gene Name:POGLUT1��
UniProt Entry Name:PGLT1_HUMAN
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